Overview

  • Product name
  • Description
    Goat polyclonal to CCR7
  • Host species
    Goat
  • Specificity
    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region. The antibody has been tested in an academic lab on cells transfected with CCR7 receptor, we do not have information regarding its use against endogenous protein.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    DPGKPRKNVLVVALLVIFQVC

    , corresponding to amino acids 2-22 of Mouse CCR7.

  • Positive control
    • IHC-P: Mouse spleen, human spleen. WB: cells transfected with CCR7 receptor

Properties

Applications

Our Abpromise guarantee covers the use of ab1657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100000.
Flow Cyt 1/10.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/250.
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • Tissue specificity
    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLR 2 antibody
    • BLR2 antibody
    • C C chemokine receptor type 7 antibody
    • C C CKR 7 antibody
    • C-C chemokine receptor type 7 antibody
    • C-C CKR-7 antibody
    • CC chemokine receptor 7 antibody
    • CC chemokine receptor type 7 antibody
    • CC CKR 7 antibody
    • CC-CKR-7 antibody
    • CCCKR7 antibody
    • CCR 7 antibody
    • CCR-7 antibody
    • Ccr7 antibody
    • CCR7_HUMAN antibody
    • CD 197 antibody
    • CD197 antibody
    • CD197 antigen antibody
    • CDw197 antibody
    • Chemokine (C C motif) receptor 7 antibody
    • Chemokine (C C) receptor 7 antibody
    • Chemokine C C motif receptor 7 antibody
    • Chemokine C C receptor 7 antibody
    • Chemokine receptor 7 antibody
    • Chemokine receptor 7-like protein antibody
    • CMKBR7 antibody
    • EBI 1 antibody
    • EBI1 antibody
    • Ebi1h antibody
    • EBV Induced G Protein Coupled Receptor 1 antibody
    • EBV-induced G-protein coupled receptor 1 antibody
    • Epstein Barr virus induced G protein coupled receptor antibody
    • Epstein Barr virus induced gene 1 antibody
    • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
    • MGC108519 antibody
    • MIP 3 beta receptor antibody
    • MIP-3 beta receptor antibody
    • MIP3 Beta Receptor antibody
    see all

Images

  • ab1657, at a dilution of 1/250, staining CCR7 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-CCR7 antibody (ab1657) at 1 µg/ml + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 20 minutes

References

This product has been referenced in:
  • Yang J  et al. Enhanced activity of macrophage M1/M2 phenotypes in periodontitis. Arch Oral Biol 96:234-242 (2018). Read more (PubMed: 28351517) »
  • Salim SY  et al. CD83+CCR7- dendritic cells accumulate in the subepithelial dome and internalize translocated Escherichia coli HB101 in the Peyer's patches of ileal Crohn's disease. Am J Pathol 174:82-90 (2009). IHC-P ; Human . Read more (PubMed: 19095953) »
See all 9 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 19 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen, lymph node)
Specification
spleen, lymph node
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Mr. Jong Hyung Lim

Verified customer

Submitted Nov 12 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
methanol/hydrogen peroxide (and I also tried 10% BSA) as blocking agent for 5 minute(s) · Concentration: 2.5%

Abcam user community

Verified customer

Submitted May 30 2007

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Specification
spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%

Dr. Linda Svantesson

Verified customer

Submitted Nov 17 2006

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