Recombinant Anti-CCR7 antibody [E75] (ab32075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E75] to CCR7
- Suitable for: ELISA, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-CCR7 antibody [E75]
See all CCR7 primary antibodies -
Description
Rabbit monoclonal [E75] to CCR7 -
Host species
Rabbit -
Specificity
ab32075 recognises CCR7/CKR7. It does not cross react with other CKR family members -
Tested Applications & Species
Application Species ELISA Recombinant fragmentWB MouseRatHuman -
Immunogen
A synthetic peptide corresponding to residues in loop-3 extracellular domain of human C-C chemokine receptor type 7
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Positive control
- Daudi cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
E75 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ELISA |
Recombinant fragment
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WB |
Mouse
Rat
Human
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All applications |
Cow
Pig
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Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
Tissue specificity
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 510668 Cow
- Entrez Gene: 1236 Human
- Entrez Gene: 12775 Mouse
- Entrez Gene: 396663 Pig
- Entrez Gene: 287673 Rat
- Omim: 600242 Human
- SwissProt: Q5MD62 Cow
- SwissProt: P32248 Human
see all -
Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
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All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : Human lung lysate
Lane 3 : Human lymph node lysate
Lane 4 : Human spleen lysate
Lane 5 : Human plasma lysate
Lane 6 : Human brain lysate (low/negative expression)
Lane 7 : Human liver lysate (low/negative expression)
Lane 8 : Mouse lung lysate
Lane 9 : Mouse lymph node lysate
Lane 10 : Mouse spleen lysate
Lane 11 : Mouse plasma lysate
Lane 12 : Rat lung lysate
Lane 13 : Rat lymph node
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab32075 has been referenced in 1 publication.
- Lu M et al. Inhibition of p21-activated kinase 1 attenuates the cardinal features of asthma through suppressing the lymph node homing of dendritic cells. Biochem Pharmacol 154:464-473 (2018). PubMed: 29906467