Recombinant
RabMAb

Anti-CCR7 antibody [E75] (ab32075)

Submit a review Q&A (2)References (1)

Overview

  • Product name
    Anti-CCR7 antibody [E75]
    See all CCR7 primary antibodies
  • Description
    Rabbit monoclonal [E75] to CCR7
  • Host species
    Rabbit
  • Specificity
    ab32075 recognises CCR7/CKR7. It does not cross react with other CKR family members
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC or IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    A synthetic peptide corresponding to residues in loop-3 extracellular domain of human C-C chemokine receptor type 7

  • Positive control
    • Daudi cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC or IP.

    • Target

    • Function
      Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
    • Tissue specificity
      Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    Images

    References

    This product has been referenced in:
    • Lu M  et al. Inhibition of p21-activated kinase 1 attenuates the cardinal features of asthma through suppressing the lymph node homing of dendritic cells. Biochem Pharmacol 154:464-473 (2018). Read more (PubMed: 29906467) »
    See 1 Publication for this product

    Publishing research using ab32075? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Dear Sir/Madam,
    I'm interested inuse the Anti-CCR7 antibody [E75] (ab32075) on porcine cells butI couldn't find any refferences about their use on porcine cells in spite of the fact that the antibody shold crossreact according to the sequence homology. Do you have any information about that, please.
    If not, would it be possible to ask you for providing me a sample of the antibody.
    Thank you in advancefor your responsiveness.
    Yours faithfully.

    Read More
    Answer

    Thank you for contacting us.

    This antibody (ab32075) has not been tested in porcine species, but it’s very likely it will cross react with this species, due to sequence homology. It is therefore stated as predicted to react in the datasheet.

    If you are interested in testing ab32075 with porcine cells with WB or Flow Cytometry, I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

    Therefore, I can offer a discount off a future purchase if you buy ab32075 and test it in porcine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab32075 with pig samples. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

    3. Test it in WB or Flow Cytometry with porcine cells.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I’m working on pig immunology and would like to use the human/mouse CCR7 ab.

    I know abcam website predict cross reactivity with pig.

    Could you please give me the sequence homology of the epitopes (%)?

    Otherwise, I know abcam is not used to provide samples but could you please offer me that in order to test the staining of my pig dendritic cells?

    That would be useful for me and for you!

    Moreover it just avoid me to waste money if the ab is finally not working but I will for sure order the product if it’s working!

    Read More
    Answer

    Thank you very much for your interest in ab32075.

    To our knowledge, ab32075 has not been tested in pig. However, the immunogen sequence shares 100% sequence homology with pig so we would expect to see cross-reactivity with pig. Therefore, I can offer a discount off a future purchase if you buy ab32075 now, test it in pig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab32075 in pig. I will then send a discount code. This code must be issued before purchasing ab32075 so please wait for my reply before ordering.

    2. Purchase ab32075 either by phone, fax, or online (www.abcam.com).

    3. Test it in pig.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab32075 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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