RecombinantRabMAb

Recombinant Anti-CCR7 antibody [E75] (ab32075)

Submit a review Q&A (2)References (1)
Western blot - Anti-CCR7 antibody [E75] (ab32075)
  • Western blot - Anti-CCR7 antibody [E75] (ab32075)
  • ELISA - Anti-CCR7 antibody [E75] (ab32075)
  • Anti-CCR7 antibody [E75] (ab32075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E75] to CCR7
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-CCR7 antibody [E75]
    See all CCR7 primary antibodies

  • Description

    Rabbit monoclonal [E75] to CCR7

  • Host species

    Rabbit

  • Specificity

    ab32075 recognises CCR7/CKR7. It does not cross react with other CKR family members

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in loop-3 extracellular domain of human C-C chemokine receptor type 7

  • Positive control

    • Daudi cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ELISA
Recombinant fragment
WB
Mouse
Rat
Human
All applications
Cow
Pig
Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
1/500. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
Notes
ELISA
Use at an assay dependent concentration.
WB
1/500. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC or IP.

    • Target

    • Function

      Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.

    • Tissue specificity

      Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    Images

    • Western blot - Anti-CCR7 antibody [E75] (ab32075)
      Western blot - Anti-CCR7 antibody [E75] (ab32075)
      All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : Human lung lysate
      Lane 3 : Human lymph node lysate
      Lane 4 : Human spleen lysate
      Lane 5 : Human plasma lysate
      Lane 6 : Human brain lysate (low/negative expression)
      Lane 7 : Human liver lysate (low/negative expression)
      Lane 8 : Mouse lung lysate
      Lane 9 : Mouse lymph node lysate
      Lane 10 : Mouse spleen lysate
      Lane 11 : Mouse plasma lysate
      Lane 12 : Rat lung lysate
      Lane 13 : Rat lymph node

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-CCR7 antibody [E75] (ab32075)
      Western blot - Anti-CCR7 antibody [E75] (ab32075)
      Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate

      Predicted band size: 43 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    • ELISA - Anti-CCR7 antibody [E75] (ab32075)
      ELISA - Anti-CCR7 antibody [E75] (ab32075)
      ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-CCR7 antibody [E75] (ab32075)
      Anti-CCR7 antibody [E75] (ab32075)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab32075? Please let us know so that we can cite the reference in this datasheet.

    ab32075 has been referenced in 1 publication.

    • Lu M  et al. Inhibition of p21-activated kinase 1 attenuates the cardinal features of asthma through suppressing the lymph node homing of dendritic cells. Biochem Pharmacol 154:464-473 (2018). PubMed: 29906467

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Dear Sir/Madam,
    I'm interested inuse the Anti-CCR7 antibody [E75] (ab32075) on porcine cells butI couldn't find any refferences about their use on porcine cells in spite of the fact that the antibody shold crossreact according to the sequence homology. Do you have any information about that, please.
    If not, would it be possible to ask you for providing me a sample of the antibody.
    Thank you in advancefor your responsiveness.
    Yours faithfully.

    Read More
    Answer

    Thank you for contacting us.

    This antibody (ab32075) has not been tested in porcine species, but it’s very likely it will cross react with this species, due to sequence homology. It is therefore stated as predicted to react in the datasheet.

    If you are interested in testing ab32075 with porcine cells with WB or Flow Cytometry, I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

    Therefore, I can offer a discount off a future purchase if you buy ab32075 and test it in porcine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab32075 with pig samples. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

    3. Test it in WB or Flow Cytometry with porcine cells.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I’m working on pig immunology and would like to use the human/mouse CCR7 ab.

    I know abcam website predict cross reactivity with pig.

    Could you please give me the sequence homology of the epitopes (%)?

    Otherwise, I know abcam is not used to provide samples but could you please offer me that in order to test the staining of my pig dendritic cells?

    That would be useful for me and for you!

    Moreover it just avoid me to waste money if the ab is finally not working but I will for sure order the product if it’s working!

    Read More
    Answer

    Thank you very much for your interest in ab32075.

    To our knowledge, ab32075 has not been tested in pig. However, the immunogen sequence shares 100% sequence homology with pig so we would expect to see cross-reactivity with pig. Therefore, I can offer a discount off a future purchase if you buy ab32075 now, test it in pig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab32075 in pig. I will then send a discount code. This code must be issued before purchasing ab32075 so please wait for my reply before ordering.

    2. Purchase ab32075 either by phone, fax, or online (www.abcam.com).

    3. Test it in pig.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab32075 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

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