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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)
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RecombinantRabMAb

Recombinant Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
  • Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
  • ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
  • Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E75] to CCR7 - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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Conjugation
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Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)

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Overview

  • Product name

    Anti-CCR7 antibody [E75] - BSA and Azide free
    See all CCR7 primary antibodies
  • Description

    Rabbit monoclonal [E75] to CCR7 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognises CCR7/CKR7. It does not cross react with other CKR family members.

  • Tested applications

    Suitable for: ELISA, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247223 is the carrier-free version of ab32075. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247223 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    E75
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Alternative Versions

    • Anti-CCR7 antibody [E75] (ab32075)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Daudi whole cell lysate (ab3951)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC or IP.
  • Target

    • Function

      Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
    • Tissue specificity

      Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P32248 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 510668 Cow
      • Entrez Gene: 1236 Human
      • Entrez Gene: 12775 Mouse
      • Entrez Gene: 396663 Pig
      • Entrez Gene: 287673 Rat
      • Omim: 600242 Human
      • SwissProt: Q5MD62 Cow
      • SwissProt: P32248 Human
      • SwissProt: P47774 Mouse
      • Unigene: 370036 Human
      • Unigene: 2932 Mouse
      see all
    • Alternative names

      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    Images

    • Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
      Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
      All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : Human lung lysate
      Lane 3 : Human lymph node lysate
      Lane 4 : Human spleen lysate
      Lane 5 : Human plasma lysate
      Lane 6 : Human brain lysate (low/negative expression)
      Lane 7 : Human liver lysate (low/negative expression)
      Lane 8 : Mouse lung lysate
      Lane 9 : Mouse lymph node lysate
      Lane 10 : Mouse spleen lysate
      Lane 11 : Mouse plasma lysate
      Lane 12 : Rat lung lysate
      Lane 13 : Rat lymph node tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      This data was developed using ab32075, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
      Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
      Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate

      Predicted band size: 43 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      This data was developed using ab32075, the same antibody clone in a different buffer formulation.

       

    • ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
      ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

      This data was developed using ab32075, the same antibody clone in a different buffer formulation.

      ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
      Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab247223? Please let us know so that we can cite the reference in this datasheet.

    ab247223 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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