Recombinant Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
![Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)](https://www.abcam.com/ps/products/247/ab247223/Images/ab247223-403561-anti-ccr7-antibody-e75-bsa-and-azide-free-western-blot-human-lung-lymph-node-spleen-plasma-brain-mouse-lung-lymph-node-spleen-plasma-rat-lung-lymph-node.jpg "Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E75] to CCR7 - BSA and Azide free
- Suitable for: ELISA, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-CCR7 antibody [E75] - BSA and Azide free
See all CCR7 primary antibodies -
Description
Rabbit monoclonal [E75] to CCR7 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognises CCR7/CKR7. It does not cross react with other CKR family members.
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Tested applications
Suitable for: ELISA, WBmore details
Unsuitable for: Flow Cyt,ICC,IHC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247223 is the carrier-free version of ab32075. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247223 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
E75 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
Tissue specificity
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 510668 Cow
- Entrez Gene: 1236 Human
- Entrez Gene: 12775 Mouse
- Entrez Gene: 396663 Pig
- Entrez Gene: 287673 Rat
- Omim: 600242 Human
- SwissProt: Q5MD62 Cow
- SwissProt: P32248 Human
see all -
Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
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Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : Human lung lysate
Lane 3 : Human lymph node lysate
Lane 4 : Human spleen lysate
Lane 5 : Human plasma lysate
Lane 6 : Human brain lysate (low/negative expression)
Lane 7 : Human liver lysate (low/negative expression)
Lane 8 : Mouse lung lysate
Lane 9 : Mouse lymph node lysate
Lane 10 : Mouse spleen lysate
Lane 11 : Mouse plasma lysate
Lane 12 : Rat lung lysate
Lane 13 : Rat lymph node tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab32075, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)](https://www.abcam.com/ps/products/247/ab247223/Images/ab247223-397858-anti-ccr7-antibody-e75-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab32075, the same antibody clone in a different buffer formulation.
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![ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)](https://www.abcam.com/ps/products/247/ab247223/Images/ab247223-3-anti-ccr7-antibody-e75-elisa-human-ccr7.jpg)
ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)This data was developed using ab32075, the same antibody clone in a different buffer formulation.
ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)](https://www.abcam.com/ps/products/247/ab247223/Images/ab247223-4-benefits-of-recombinant-antibodies.png)
Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab247223 has not yet been referenced specifically in any publications.