Recombinant Anti-CCR7 antibody [EPR23192-57] (ab253187)
![Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-8-anti-ccr7-antibody-epr2319257-immunohistochemistry-frozen-mouse-pancreas.jpg "Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23192-57] to CCR7
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CCR7 antibody [EPR23192-57]
See all CCR7 primary antibodies -
Description
Rabbit monoclonal [EPR23192-57] to CCR7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Unsuitable for: Flow Cyt,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, Human Hodgkin's lymphoma, Mouse spleen and Rat spleen tissues. IHC-Fr: Mouse spleen and Rat spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23192-57 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20). |
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
Tissue specificity
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1236 Human
- Entrez Gene: 12775 Mouse
- Entrez Gene: 287673 Rat
- Omim: 600242 Human
- SwissProt: P32248 Human
- SwissProt: P47774 Mouse
- Unigene: 370036 Human
- Unigene: 2932 Mouse
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Alternative names
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling CCR7 with ab253187 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: No staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
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![Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-7-anti-ccr7-antibody-epr2319257-immunohistochemistry-frozen-rat-spleen.jpg)
Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
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![Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-6-anti-ccr7-antibody-epr2319257-immunohistochemistry-frozen-mouse-spleen.jpg)
Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).<\p>
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-5-anti-ccr7-antibody-epr2319257-immunohistochemistry-human-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human pancreas. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-4-anti-ccr7-antibody-epr2319257-immunohistochemistry-rat-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat spleen. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-3-anti-ccr7-antibody-epr2319257-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-2-anti-ccr7-antibody-epr2319257-immunohistochemistry-human-hodgkins-lymphoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on RS cells in human Hodgkin's lymphoma. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-1-anti-ccr7-antibody-epr2319257-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (ab253187)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human tonsil. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Anti-CCR7 antibody [EPR23192-57] (ab253187)](https://www.abcam.com/ps/products/253/ab253187/Images/ab253187-9-benefits-of-recombinant-antibodies.png)
Anti-CCR7 antibody [EPR23192-57] (ab253187)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab253187 has been referenced in 2 publications.
- Montanaro M et al. The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification is Correlated with Plaque Instability. Int J Mol Sci 22:N/A (2021). PubMed: 33401449
- Dong R et al. Electrospun polycaprolactone (PCL)-amnion nanofibrous membrane prevents adhesions and promotes nerve repair in a rat model of sciatic nerve compression. PLoS One 15:e0244301 (2020). PubMed: 33338083
