Recombinant
RabMAb

Anti-CCR7 antibody [Y59] (ab32527)

Overview

  • Product name
    Anti-CCR7 antibody [Y59]
    See all CCR7 primary antibodies
  • Description
    Rabbit monoclonal [Y59] to CCR7
  • Host species
    Rabbit
  • Specificity
    The antibody does not cross-react with other G-protein coupled receptor 1 family members.
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human CCR7 (N terminal). The exact sequence is proprietary.
    Database link: P32248
    (Peptide available as ab209386)

  • Positive control
    • WB: MCF7, K562, Daudi, COS-1, C6, RAW 264.7 and PC-12 cell lysates. ICC/IF: Jurkat and HeLa cells. IP: MCF7 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with CCR7 peptide (ab209386).

For unpurified use at 1/5000.

ICC/IF 1/100.

For unpurified use at 1/250.

IP 1/20.

For unpurified use at 1/10.

Target

  • Function
    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • Tissue specificity
    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLR 2 antibody
    • BLR2 antibody
    • C C chemokine receptor type 7 antibody
    • C C CKR 7 antibody
    • C-C chemokine receptor type 7 antibody
    • C-C CKR-7 antibody
    • CC chemokine receptor 7 antibody
    • CC chemokine receptor type 7 antibody
    • CC CKR 7 antibody
    • CC-CKR-7 antibody
    • CCCKR7 antibody
    • CCR 7 antibody
    • CCR-7 antibody
    • Ccr7 antibody
    • CCR7_HUMAN antibody
    • CD 197 antibody
    • CD197 antibody
    • CD197 antigen antibody
    • CDw197 antibody
    • Chemokine (C C motif) receptor 7 antibody
    • Chemokine (C C) receptor 7 antibody
    • Chemokine C C motif receptor 7 antibody
    • Chemokine C C receptor 7 antibody
    • Chemokine receptor 7 antibody
    • Chemokine receptor 7-like protein antibody
    • CMKBR7 antibody
    • EBI 1 antibody
    • EBI1 antibody
    • Ebi1h antibody
    • EBV Induced G Protein Coupled Receptor 1 antibody
    • EBV-induced G-protein coupled receptor 1 antibody
    • Epstein Barr virus induced G protein coupled receptor antibody
    • Epstein Barr virus induced gene 1 antibody
    • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
    • MGC108519 antibody
    • MIP 3 beta receptor antibody
    • MIP-3 beta receptor antibody
    • MIP3 Beta Receptor antibody
    see all

Images

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : COS-1 (african green monkey kidney fibroblast-like cell line) cell lysate
    Lane 2 : C6 (rat glial tumor cell line) cell lysate
    Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 43 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of Jurkat (human  T cell leukemia cell line from peripheral blood) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF7 (human breast adenocarcinoma cell line) whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 3 : Daudi (human Burkitt's lymphoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 43 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling CCR7 with unpurified ab32527 at 1/1000. Cells were fixed with 4% PFA fixed (10 min) and permeabilized and blocked with 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (shown in green). Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilution (unpurified)

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 43 kDa
    Observed band size: 48 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Prest TA  et al. Nerve-specific, xenogeneic extracellular matrix hydrogel promotes recovery following peripheral nerve injury. J Biomed Mater Res A 106:450-459 (2018). Read more (PubMed: 28891122) »
  • Basheer HA  et al. Hypoxia modulates CCR7 expression in head and neck cancers. Oral Oncol 80:64-73 (2018). Read more (PubMed: 29706190) »

See all 27 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Abdominal Aorta)
Permeabilization
No
Specification
Abdominal Aorta
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
200 Proof Ethyl Alcohol
Username

Abcam user community

Verified customer

Submitted Mar 21 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Gel Running Conditions
Reduced Denaturing
Loading amount
7 µg
Specification
MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (CT26-WT tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
CT26-WT tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Miss. Sylvie Colin

Verified customer

Submitted Jun 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Sample
Rat Tissue sections (nerve)
Specification
nerve
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 11 2013

Thank you very much for contacting us.

Rabbit monoclonal antibodies have very high affinity for their antigens, so they are often used more diluted than a comparable mouse monoclonal antibody would be. We don't have any data for htis antibody...

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Thanks for the email.

I'm happy to provide the concentration. It's lot specific so when you have a chance please provide the lot number for that vial of ab32527.

Thanks!

Thanks for your email.

According to information I have this product should arrive as a liquid (100ul). I'm sorry to ask a rather silly question here but did you centrifuge the vial prior to opening, in case all the liquid is in the cap?
...

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Thanks for your email.

I hope you've been able to claim your free antibody. If not, please note I have added your discount code to your review for ab32527 so you should have no problem using that code to get your free primary now.

I...

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Application
Flow Cytometry
Sample
Rat Cell (primary cortical cultures)
Specification
primary cortical cultures
Fixation
none
Permeabilization
No
Gating Strategy
live cells (no PI uptake)
Username

Abcam user community

Verified customer

Submitted Aug 25 2012


I am very pleased to hear you would like to accept our offer and test ab32527 in flow cytometry. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an A...

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1-10 of 15 Abreviews or Q&A

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