Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y59] to CCR7 (HRP)
- Suitable for: WB
- Reacts with: Mouse, Rat
- Conjugation: HRP
Product nameAnti-CCR7 antibody [Y59] (HRP)
See all CCR7 primary antibodies
DescriptionRabbit monoclonal [Y59] to CCR7 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
Synthetic peptide within Human CCR7 (N terminal). The exact sequence is proprietary.
- WB: C6 and Raw264.7 whole cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 488) (ab196459)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 647) (ab196640)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 594) (ab207016)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 555) (ab207018)
- Anti-CCR7 antibody [Y59] (Alexa Fluor® 568) (ab207019)
- Anti-CCR7 antibody [Y59] - Low endotoxin, Azide free (ab221209)
- Anti-CCR7 antibody [Y59] (ab32527)
Our Abpromise guarantee covers the use of ab196475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
This product is not recommended for use with human samples in western blot.
FunctionReceptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Tissue specificityExpressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
All lanes : Anti-CCR7 antibody [Y59] (HRP) (ab196475) at 1/5000 dilution (Recombinant Anti-CCR7 antibody [Y59] (HRP))
Lane 1 : C6 (rat glial tumor cell line) cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Exposure time: 10 seconds
ab196475 was shown to react with CCR7 in western blot. Membranes were blocked in 2% BSA in TBS-T (0.1% Tween®) before incubation with ab196475 overnight at 4°C at a 1 in 5000 dilution. Blots were incubated with secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
ab196475 has not yet been referenced specifically in any publications.