Key features and details
- Rat monoclonal [7E7] to CCR9
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-CCR9 antibody [7E7]
See all CCR9 primary antibodies
DescriptionRat monoclonal [7E7] to CCR9
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 5% Fetal calf serum
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionReceptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Tissue specificityHighly expressed in the thymus and low in lymph nodes and spleen.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- C C chemokine receptor D6 antibody
- C C chemokine receptor type 9 antibody
- C C CKR 9 antibody
ab52590 staining CCR9 in Mouse brain tissue sections by Immunocytochemistry/ Immunofluorescence.
Red = CCR9
Green = IgA
Blue = CD3
Overlay histogram showing K562 cells stained with ab52590 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52590, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab52590 has been referenced in 1 publication.
- Pabst O et al. Chemokine receptor CCR9 contributes to the localization of plasma cells to the small intestine. J Exp Med 199:411-6 (2004). PubMed: 14744993