• Product name
  • Description
    Goat polyclonal to CCR9
  • Host species
  • Specificity
    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
  • Tested applications
    Suitable for: IHC-FoFr, Flow Cyt, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 10-37 of Mouse CCR9.

  • Positive control
    • Mouse thymus or lymphnodes.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab1662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/500 - 1/1000. After perfusion place in 30% sucrose overnight.
Flow Cyt 1/200.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.


ELISA 1/100000.
IHC-P 1/250.
WB 1/1000.


  • Relevance
    The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • C C chemokine receptor D6 antibody
    • C C chemokine receptor type 9 antibody
    • C C CKR 9 antibody
    • CC Chemokine receptor CCR10 antibody
    • CC CKR 9 antibody
    • CCBP2 antibody
    • CCCKR9 antibody
    • CCR 9 antibody
    • CCR9 antibody
    • CDw199 antibody
    • CDw199 antigen antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
    • Chemokine (C-C motif) receptor 9 isoform A antibody
    • Chemokine (C-C motif) receptor 9 isoform B antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 10 antibody
    • Chemokine CC Motif Receptor 9 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine-binding protein D6 antibody
    • Cmkbr10 antibody
    • CMKBR9 antibody
    • G protein coupled receptor 28 antibody
    • GPR 28 antibody
    • GPR 9 6 antibody
    • GPR28 antibody
    • GPR96 antibody
    • OTTHUMP00000164653 antibody
    • OTTHUMP00000164654 antibody
    see all


  • Immonuhistochemistry on mouse spleen, at 1/250 dilution.


This product has been referenced in:
  • Huang Y  et al. Abrogation of CC chemokine receptor 9 ameliorates ventricular remodeling in mice after myocardial infarction. Sci Rep 6:32660 (2016). Read more (PubMed: 27585634) »
  • Chu PS  et al. C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice. Hepatology 58:337-50 (2013). Read more (PubMed: 23460364) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your patience. The originator of ab12953 and ab13396 has gotten back to me with the standard IHC protocol that they follow which I have included below. If you have any additional questions, please let me know. Tissue Preparation: Formalin fixation and embedding in paraffin wax Tissue Sectioning: Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C. Deparaffinization: Wash dry slides in 3 changes of xylene – 5 minutes each @ RT Rehydration: Wash slides in 3 changes of 100% alcohol – 3 minutes each @ RT Wash slides in 2 changes of 95% alcohol – 3 minutes each @ RT Wash slides in 1 change of 80% alcohol – 3 minutes @ RT Rinse slides in gentle running distilled water – 5 minutes @ RT Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute @ RT Immunostaining: (Do not allow tissues to dry at any time during the staining procedure) Apply a universal protein block – 20 minutes @ RT Drain protein block from slides, apply diluted primary antibody – 45 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply a biotinylated anti-rabbit IgG (H+L) secondary – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase streptavidin – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase chromogen substrate – 30 minutes @ RT Wash slides in distilled water – 1 minute @ RT Dehydrate: (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) Wash slides in 2 changes of 80% alcohol – 1 minute each @ RT Wash slides in 2 changes of 95% alcohol – 1 minute each @ RT Wash slides in 3 changes of 100% alcohol – 1 minute each @ RT Wash slides in 3 changes of xylene – 1 minute each @ RT Apply coverslip

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Thank you for your phone call. For ab13268, ab1662, and ab1669, the originator has informed me that the antigen retrieval method for IHC-formalin fixed, paraffin embedded sections involved treating the sections with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. I will contact you again when I receive information regarding ab12953 and ab13396. Have a great weekend!

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