Product nameAnti-CCR9 antibody [E99]
See all CCR9 primary antibodies
DescriptionRabbit monoclonal [E99] to CCR9
SpecificityThe antibody is specific to CCR9. It does not cross react with other G protein coupled receptor 1 family members.
Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
Unsuitable for: IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CCR9 (N terminal). The exact sequence is proprietary. A synthetic peptide corresponding to an extracellular domain of human CCR9.
- K562 and Daudi cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
Cellular localizationCell membrane; Multi-pass membrane protein.
- C C chemokine receptor D6 antibody
- C C chemokine receptor type 9 antibody
- C C CKR 9 antibody
All lanes : Anti-CCR9 antibody [E99] (ab32556) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : Daudi cell lysate
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Chen X et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . Read more (PubMed: 27080036) »
- Maeda S et al. Molecular cloning and expression analysis of the canine chemokine receptor CCR9. Vet Immunol Immunopathol 145:534-9 (2012). WB . Read more (PubMed: 22209203) »