CCRF-CEM nuclear extract lysate (ab14642)

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Overview

  • Product name

    CCRF-CEM nuclear extract lysate

  • General notes

    We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • Research areas

  • Background

    The direct isolation and propagation of a continuous culture of human lymphoblasts (CCRF-CEM) (also called CEM) from the peripheral blood of a patient with acute lymphoblastic leukemia has permitted extensive sequential cytogenetic analysis of these cells during continuous propagation in culture.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14642? Please let us know so that we can cite the reference in this datasheet.

ab14642 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Do you have karyotype info on the CCRFCEM line that these nuclear lysates came from? I have been working with CEM-C7 and CEM C1 cells and would like to utilitze your lysate as a positive control, but want to make sure the karyotype is the same.

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Answer

Thank you for your enquiry. This extract was prepared from the parental cell line CCRF-CEM (ATCC number CCL-119) Karyotype: modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted. I hope this information will help you, please let me know if I can be of further assistance,

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Question

Ab14642 is listed as being nuclear lysate, but on the datasheet under purity it states whole cell lysate. Please clarify.

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Answer

Thank you for your phone call. I have confirmed that ab14642 is CCRF-CEM nuclear extract, and have updated the online datasheet. We unfortunately do not currently carry CCRF-CEM whole cell lysate. If you have any additional questions, please contact us again.

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