CCRF-CEM nuclear extract lysate (ab14642)

Q&A (2)

Overview

  • Product name
    CCRF-CEM nuclear extract lysate
  • General notes
    We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    The direct isolation and propagation of a continuous culture of human lymphoblasts (CCRF-CEM) (also called CEM) from the peripheral blood of a patient with acute lymphoblastic leukemia has permitted extensive sequential cytogenetic analysis of these cells during continuous propagation in culture.

References

ab14642 has not yet been referenced specifically in any publications.

Publishing research using ab14642? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
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1-2 of 2 Q&A

Question

Do you have karyotype info on the CCRFCEM line that these nuclear lysates came from? I have been working with CEM-C7 and CEM C1 cells and would like to utilitze your lysate as a positive control, but want to make sure the karyotype is the same.

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Answer

Thank you for your enquiry. This extract was prepared from the parental cell line CCRF-CEM (ATCC number CCL-119) Karyotype: modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted. I hope this information will help you, please let me know if I can be of further assistance,

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Question

Ab14642 is listed as being nuclear lysate, but on the datasheet under purity it states whole cell lysate. Please clarify.

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Answer

Thank you for your phone call. I have confirmed that ab14642 is CCRF-CEM nuclear extract, and have updated the online datasheet. We unfortunately do not currently carry CCRF-CEM whole cell lysate. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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