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Do you have karyotype info on the CCRFCEM line that these nuclear lysates came from? I have been working with CEM-C7 and CEM C1 cells and would like to utilitze your lysate as a positive control, but want to make sure the karyotype is the same.
Asked on Jan 22 2007
Thank you for your enquiry. This extract was prepared from the parental cell line CCRF-CEM (ATCC number CCL-119) Karyotype: modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted. I hope this information will help you, please let me know if I can be of further assistance,
Answered on Jan 25 2007