Overview

  • Product name
  • Description
    Rabbit polyclonal to CCRK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment within Human CCRK (internal sequence). The exact sequence is proprietary.
    Database link: Q8IZL9

  • Positive control
    • WB: HepG2 whole cell lysate. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Required for high-level Shh responses in the developing neural tube. Together with BROMI, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling (By similarity). Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus. Cytoplasm. Cell projection > cilium.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAK kinase p42 antibody
    • CAK-kinase p42 antibody
    • CCRK antibody
    • CDCH antibody
    • CDK activating kinase p42 antibody
    • CDK-activating kinase p42 antibody
    • cdk20 antibody
    • CDK20_HUMAN antibody
    • Cell cycle related kinase antibody
    • Cell cycle-related kinase antibody
    • Cell division protein kinase 20 antibody
    • Cyclin dependent protein kinase H antibody
    • Cyclin kinase activating kinase p42 antibody
    • Cyclin-dependent kinase 20 antibody
    • Cyclin-dependent protein kinase H antibody
    • Cyclin-kinase-activating kinase p42 antibody
    • p42 antibody
    • PNQALRE antibody
    see all

Images

  • Anti-CCRK antibody (ab227077) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 39 kDa

  • 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CCRK (green) using ab227077 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

References

This product has been referenced in:
  • Yu Z  et al. Bufalin suppresses hepatocarcinogenesis by targeting ß-catenin/TCF signaling via cell cycle-related kinase. Sci Rep 8:3891 (2018). Read more (PubMed: 29497076) »
See 1 Publication for this product

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