Key features and details
- Rabbit polyclonal to CCRK
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CCRK antibody
See all CCRK primary antibodies
DescriptionRabbit polyclonal to CCRK
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee
Recombinant fragment within Human CCRK (internal sequence). The exact sequence is proprietary.
Database link: Q8IZL9
- WB: HepG2 whole cell lysate. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 39 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionRequired for high-level Shh responses in the developing neural tube. Together with BROMI, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling (By similarity). Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Cellular localizationNucleus. Cytoplasm. Cell projection > cilium.
- Information by UniProt
- CAK kinase p42 antibody
- CAK-kinase p42 antibody
- CCRK antibody
Anti-CCRK antibody (ab227077) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CCRK (green) using ab227077 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227077 has been referenced in 2 publications.
- Wang R et al. Androgen Receptor Promotes Gastric Carcinogenesis via Upregulating Cell Cycle-Related Kinase Expression. J Cancer 10:4178-4188 (2019). PubMed: 31413736
- Yu Z et al. Bufalin suppresses hepatocarcinogenesis by targeting ß-catenin/TCF signaling via cell cycle-related kinase. Sci Rep 8:3891 (2018). PubMed: 29497076