Product nameAnti-CCT2 antibody [EPR4083]
See all CCT2 primary antibodies
DescriptionRabbit monoclonal [EPR4083] to CCT2
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CCT2 aa 1-100. The exact sequence is proprietary.
- HeLa, MCF-7 and A431 cell lysates; Human kidney tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 57 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
- Information by UniProt
- 99D8.1 antibody
- CCT 2 antibody
- CCT beta antibody
All lanes : Anti-CCT2 antibody [EPR4083] (ab109184) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
ab109184, at 1/100 dilution, staining CCT2 in Human kidney tissue by Immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109184 has not yet been referenced specifically in any publications.