Key features and details
- Rabbit polyclonal to CCT3
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CCT3 antibody
See all CCT3 primary antibodies
DescriptionRabbit polyclonal to CCT3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, Jurkat, 293T and Mouse NIH3T3 cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 61 kDa.|
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
- Information by UniProt
- CCT 3 antibody
- CCT gamma antibody
- CCT-gamma antibody
Detection of Human CCT3 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176686 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes : Anti-CCT3 antibody (ab176686) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 3 seconds
ab176686 has not yet been referenced specifically in any publications.