Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Detection of Human CCT3 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176686 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western blot - Anti-CCT3 antibody (ab176686)
All lanes : Anti-CCT3 antibody (ab176686) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : 293T cell lysate at 50 µg Lane 4 : Jurkat cell lysate at 50 µg Lane 5 : Mouse NIH3T3 cell lysate at 50 µg