Overview

  • Product name

  • Description

    Rabbit polyclonal to CCT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide within Human CCT3 aa 300-350. The exact sequence is proprietary. (NP_005989.3).
    Database link: P49368

  • Positive control

    • IHC-P: Human lung carcinoma tissue; Mouse teratoma tissue. WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225878 was affinity purified using an epitope specific to CCT3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Sequence similarities

    Belongs to the TCP-1 chaperonin family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCT 3 antibody
    • CCT gamma antibody
    • CCT-gamma antibody
    • CCT3 antibody
    • CCTG antibody
    • Chaperonin containing TCP1 subunit 3 (gamma) antibody
    • Chaperonin containing TCP1 subunit 3 antibody
    • hTRiC5 antibody
    • PIG48 antibody
    • T complex protein 1 gamma subunit antibody
    • T complex protein 1 subunit gamma antibody
    • T-complex protein 1 subunit gamma antibody
    • TCP 1 gamma antibody
    • TCP-1-gamma antibody
    • TCP1 (t complex 1) ring complex polypeptide 5 antibody
    • TCPG_HUMAN antibody
    • TRIC5 antibody
    see all

Images

  • All lanes : Anti-CCT3 antibody (ab225878) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa


    Exposure time: 1 second
  • Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

  • CCT3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225878 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225878 at 0.4 µg/ml.

    Lane 1: ab225878 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 1 second.

  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

References

ab225878 has not yet been referenced specifically in any publications.

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