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Synthetic peptide within Human CCT3 aa 515-545 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P49368
Our Abpromise guarantee covers the use of ab174255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 61 kDa.|
Flow cytometric analysis of K562 cells labeling CCT3 with ab174255 at 1/10 (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling CCT3 with ab174255 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"