Overview

  • Product name
    Anti-CCT3 antibody - C-terminal
    See all CCT3 primary antibodies
  • Description
    Rabbit polyclonal to CCT3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Monkey
  • Immunogen

    Synthetic peptide within Human CCT3 aa 515-545 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P49368

  • Positive control
    • K562 cells and cell line lysates; Human lung carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174255 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.

Target

  • Function
    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Sequence similarities
    Belongs to the TCP-1 chaperonin family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCT 3 antibody
    • CCT gamma antibody
    • CCT-gamma antibody
    • CCT3 antibody
    • CCTG antibody
    • Chaperonin containing TCP1 subunit 3 (gamma) antibody
    • Chaperonin containing TCP1 subunit 3 antibody
    • hTRiC5 antibody
    • PIG48 antibody
    • T complex protein 1 gamma subunit antibody
    • T complex protein 1 subunit gamma antibody
    • T-complex protein 1 subunit gamma antibody
    • TCP 1 gamma antibody
    • TCP-1-gamma antibody
    • TCP1 (t complex 1) ring complex polypeptide 5 antibody
    • TCPG_HUMAN antibody
    • TRIC5 antibody
    see all

Images

  • Flow cytometric analysis of K562 cells labeling CCT3 with ab174255 at 1/10 (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling CCT3 with ab174255 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-CCT3 antibody - C-terminal (ab174255) at 1/100 dilution

    Lane 1 : K562 cell line lysates with no blocking peptide
    Lane 2 : K562 cell line lysates with blocking peptide

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 61 kDa

References

This product has been referenced in:
  • Shi X  et al. Suppression of CCT3 inhibits malignant proliferation of human papillary thyroid carcinoma cell. Oncol Lett 15:9202-9208 (2018). Read more (PubMed: 29805652) »
  • Li LJ  et al. Chaperonin containing TCP-1 subunit 3 is critical for gastric cancer growth. Oncotarget 8:111470-111481 (2017). Read more (PubMed: 29340068) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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