- Datasheet
- Specific References (26)
- Protocols
Overview
-
Product nameAnti-CD10 antibody [56C6]
See all CD10 primary antibodies -
DescriptionMouse monoclonal [56C6] to CD10
-
Host speciesMouse
-
Tested applicationsSuitable for: WB, IHC-Pmore details
-
Species reactivityReacts with: Cat, Dog, Human
Predicted to work with: Mouse
-
Immunogen
Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein
-
Positive control
- IHC-P: MDA-MB-231 human breast cancer cells grown in mouse. Renal cell carcinoma tissue.
-
General notes
We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.
This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Renoir Red Diluent, pH 7.3 -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone number56C6
-
IsotypeIgG1
-
Research areas
Associated products
-
Antibody Diluent
-
Compatible Secondaries
-
Unmasking reagent
Applications
Our Abpromise guarantee covers the use of ab951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 85 kDa. PubMed: 26074811 | |
| IHC-P | 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-). |
Target
-
FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
-
Sequence similaritiesBelongs to the peptidase M13 family.
-
Cellular localizationCell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- Unigene: 307734 Human
- Unigene: 296022 Mouse
-
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)](https://www.abcam.com/ps/products/0/ab951/Images/ab951-284423-ab9513331.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)Paraffin embedded renal cell carcinoma tissue stained for CD10 with ab951 (1/25 dilution) in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)](https://www.abcam.com/ps/products/0/ab951/Images/CD10-Primary-antibodies-ab951-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)This image is courtesy of an anonymous Abreview.ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.
References
This product has been referenced in:
- Li X et al. Encapsulated papillary carcinoma of the breast: A clinicopathological study of 49 cases. Curr Probl Cancer N/A:N/A (2018). Read more (PubMed: 29731165) »
- Zhu L et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"