Anti-CD10 antibody (ab73409)

Rabbit polyclonal CD10 antibody. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 1 publication(s). Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to CD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to Human CD10 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab109275)

  • Positive control

    • This antibody gave a positive signal in the following lysates: LNCaP Nuclear; Ramos Whole Cell; Raji Whole Cell; Human Kidney Tissue; Human Placenta Tissue This antibody gave a positive result in IHC in the following FFPE tissue: human normal kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab73409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similarities

    Belongs to the peptidase M13 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atriopeptidase antibody
    • CALLA antibody
    • CD10 antibody
    • CD10 antigen antibody
    • Common acute lymphocytic leukemia antigen antibody
    • DKFZp686O16152 antibody
    • EC 3.4.24.11 antibody
    • Enkephalinase antibody
    • EPN antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
    • Membrane metallo endopeptidase antibody
    • Membrane metallo endopeptidase variant 1 antibody
    • Membrane metallo endopeptidase variant 2 antibody
    • Membrane metalloendopeptidase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
    • Membrane metalloendopeptidase variant 1 antibody
    • Membrane metalloendopeptidase variant 2 antibody
    • MGC126681 antibody
    • MGC126707 antibody
    • MME antibody
    • NEP antibody
    • NEP_HUMAN antibody
    • Neprilysin antibody
    • neprilysin-390 antibody
    • neprilysin-411 antibody
    • Neutral endopeptidase 24.11 antibody
    • Neutral endopeptidase antibody
    • Neutral endopeptidase, membrane-associated antibody
    • SFE antibody
    • Skin fibroblast elastase antibody
    see all

Images

  • All lanes : Anti-CD10 antibody (ab73409) at 1 µg/ml

    Lane 1 : LNCaP nuclear extract lysate (ab14857)
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)
    Lane 5 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    CD10 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab73409 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73409, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of CD10 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73409, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • El Jamal SM  et al. MEF2B is a member of the BCL6 gene transcriptional complex and induces its expression in diffuse large B-cell lymphoma of the germinal center B-cell-like type. Lab Invest N/A:N/A (2018). Read more (PubMed: 30446717) »
  • Su S  et al. CD10+GPR77+ Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness. Cell 172:841-856.e16 (2018). Read more (PubMed: 29395328) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (endometrium, uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER10min. with BOND Epitope Retrieval Solution 2 (pH: 9.0)
Permeabilization
No
Specification
endometrium, uterus
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 19 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Kidney)
Permeabilization
Yes - SDS 4%
Specification
Kidney
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2017

Answer

Indeed C-terminal is the extracellular domain so you can try this ab on unfixed cells. Do you want me to issue you a Abtrial discount code?

Read More

Answer

The antibody hasn't been tested with Rat samples; it is only predicted based on the homology of immunogen sequence between human and Rat. We currently do not hold any rat specific data.
In case you are interested in trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
I can provide you code for Flow cytometry application.
The immunogen of this product is against the C terminal end so you need to permeabilize the cell. As we haven't tested this ab in Flow cytometry so we are not sure if it is suitable for unfixed fresh cells. Please try one vial to know.

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