Overview

  • Product name

  • Description

    Rabbit polyclonal to CD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD10 aa 450-550 (internal sequence). The exact sequence is proprietary.
    Database link: P08473

  • Positive control

    • Human tonsil and human small intestine tissue. In western blot, this antibody gave a positive signal in human kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100. For antigen retrieval boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).

Target

  • Function

    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similarities

    Belongs to the peptidase M13 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atriopeptidase antibody
    • CALLA antibody
    • CD10 antibody
    • CD10 antigen antibody
    • Common acute lymphocytic leukemia antigen antibody
    • DKFZp686O16152 antibody
    • EC 3.4.24.11 antibody
    • Enkephalinase antibody
    • EPN antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
    • Membrane metallo endopeptidase antibody
    • Membrane metallo endopeptidase variant 1 antibody
    • Membrane metallo endopeptidase variant 2 antibody
    • Membrane metalloendopeptidase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
    • Membrane metalloendopeptidase variant 1 antibody
    • Membrane metalloendopeptidase variant 2 antibody
    • MGC126681 antibody
    • MGC126707 antibody
    • MME antibody
    • NEP antibody
    • NEP_HUMAN antibody
    • Neprilysin antibody
    • neprilysin-390 antibody
    • neprilysin-411 antibody
    • Neutral endopeptidase 24.11 antibody
    • Neutral endopeptidase antibody
    • Neutral endopeptidase, membrane-associated antibody
    • SFE antibody
    • Skin fibroblast elastase antibody
    see all

Images

  • ab82073, at 1/100 dilution, staining of Formalin-fixed, paraffin-embedded Human small intestine by Immunohistochemistry
  • Anti-CD10 antibody (ab82073) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    CD10 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:

  • Ruiduo C  et al. CXCL9 promotes the progression of diffuse large B-cell lymphoma through up-regulating ß-catenin. Biomed Pharmacother 107:689-695 (2018). Read more (PubMed: 30130730) »
See 1 Publication for this product

Customer reviews and Q&As

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Answer

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