Product nameAnti-CD10 antibody
See all CD10 primary antibodies
DescriptionRabbit polyclonal to CD10
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD10 aa 450-550 (internal sequence). The exact sequence is proprietary.
Database link: P08473
- Human tonsil and human small intestine tissue. In western blot, this antibody gave a positive signal in human kidney tissue lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100. For antigen retrieval boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).|
FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similaritiesBelongs to the peptidase M13 family.
Cellular localizationCell membrane.
- Information by UniProt
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
ab82073, at 1/100 dilution, staining of Formalin-fixed, paraffin-embedded Human small intestine by Immunohistochemistry
Anti-CD10 antibody (ab82073) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
CD10 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.