Recombinant Anti-CD10 antibody [EPR22865-73] (ab255609)
![Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-5-anti-cd10-epr22685-73-western-blot-human.jpg "Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22865-73] to CD10
- Suitable for: WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD10 antibody [EPR22865-73]
See all CD10 primary antibodies -
Description
Rabbit monoclonal [EPR22865-73] to CD10 -
Host species
Rabbit -
Specificity
IHC application is recommended for human only.
-
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human placenta tisssue lysate; rat kidney and liver lysates; Raji, Ramos and HAP1 whole cell lysates; rat kidney and liver lysates; mouse kidney lysate. IHC-P: Human kidney, breast, liver and tonsil tissue. ICC/IF: Raji and Ramos cells. IP: Raji whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22865-73 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Immunohistochemistry reagents
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa).
|
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC application is recommended for human only. |
|
| ICC/IF |
1/50.
|
|
| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC application is recommended for human only. |
|
ICC/IF
1/50. |
|
IP
1/30. |
Target
-
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Entrez Gene: 24590 Rat
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- SwissProt: P07861 Rat
- Unigene: 307734 Human
see all -
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
-
Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)All lanes : Anti-CD10 antibody [EPR22865-73] (ab255609) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 5 : Wild-type HAP1 whole cell lysate
Lane 6 : CD10 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?ab255609 was shown to specifically react with CD10 in wild-type HAP1 cells as signal was lost in CD10 knockout cells. Wild-type and CD10 knockout samples were subjected to SDS-PAGE. ab255609 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Lanes 5-6 in this blot were developed using a higher sensitivity ECL substrate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 10 seconds; Lanes 2-4: 15 seconds; Lanes 5-6: 70 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:15286660).
Negative control: HT-29 (PMID:19828468).
-
![Immunoprecipitation - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-10-anti-cd10-epr22685-73-immunoprecipitation-raji-human.jpg)
Immunoprecipitation - Anti-CD10 antibody [EPR22865-73] (ab255609)CD10 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab255609 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab255609 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Raji whole cell lysate 10 μg (Input).
Lane 2: ab255609 IP in Raji whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255609 in Raji whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-9-anti-cd10-epr22685-73-immunocytochemistry-ramos-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] (ab255609)Immunofluorescent analysis of 100% methanol-fixed Ramos (human Burkitt's lymphoma cell line) cells labeling CD10 with ab255609 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Ramos cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
100% methanol was preferred as fixative.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-8-anti-cd10-epr22685-73-immunocytochemistry-raji-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] (ab255609)Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling CD10 with ab255609 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
100% methanol was preferred as fixative.
-
![Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-7-anti-cd10-epr22685-73-western-blot-kidney-mouse.jpg)
Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)Anti-CD10 antibody [EPR22865-73] (ab255609) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:15286660).
-
![Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-6-anti-cd10-epr22685-73-western-blot-rat.jpg)
Western blot - Anti-CD10 antibody [EPR22865-73] (ab255609)All lanes : Anti-CD10 antibody [EPR22865-73] (ab255609) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:15286660).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-4-anti-cd10-epr22685-73-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on germinal center of human tonsil (PMID:10843287) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-3-anti-cd10-epr22685-73-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on intrahepatic canaliculi of human liver (PMID:10705818) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-2-anti-cd10-epr22685-73-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on myoepithelial cells of human breast (PMID:10705818, 17143263) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-1-anti-cd10-epr22685-73-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] (ab255609)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on proximal convoluted tubules and glomerular epithelial cells of human kidney (PMID:10705818) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-CD10 antibody [EPR22865-73] (ab255609)](https://www.abcam.com/ps/products/255/ab255609/Images/ab255609-11-benefits-of-recombinant-antibodies.png)
Anti-CD10 antibody [EPR22865-73] (ab255609)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab255609 has not yet been referenced specifically in any publications.
