Recombinant
RabMAb

Recombinant Anti-CD10 antibody [EPR2997] (ab79423)

Overview

  • Product name

    Anti-CD10 antibody [EPR2997]
    See all CD10 primary antibodies
  • Description

    Rabbit monoclonal [EPR2997] to CD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminal of Human CD10.

  • Positive control

    • WB: LNCAP, Human brain, Mouse brain, and Rat brain lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab79423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
Flow Cyt 1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
    • Sequence similarities

      Belongs to the peptidase M13 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Atriopeptidase antibody
      • CALLA antibody
      • CD10 antibody
      • CD10 antigen antibody
      • Common acute lymphocytic leukemia antigen antibody
      • DKFZp686O16152 antibody
      • EC 3.4.24.11 antibody
      • Enkephalinase antibody
      • EPN antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
      • Membrane metallo endopeptidase antibody
      • Membrane metallo endopeptidase variant 1 antibody
      • Membrane metallo endopeptidase variant 2 antibody
      • Membrane metalloendopeptidase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
      • Membrane metalloendopeptidase variant 1 antibody
      • Membrane metalloendopeptidase variant 2 antibody
      • MGC126681 antibody
      • MGC126707 antibody
      • MME antibody
      • NEP antibody
      • NEP_HUMAN antibody
      • Neprilysin antibody
      • neprilysin-390 antibody
      • neprilysin-411 antibody
      • Neutral endopeptidase 24.11 antibody
      • Neutral endopeptidase antibody
      • Neutral endopeptidase, membrane-associated antibody
      • SFE antibody
      • Skin fibroblast elastase antibody
      see all

    Images

    • All lanes : Anti-CD10 antibody [EPR2997] (ab79423) at 1/2000 dilution

      Lane 1 : LNCAP (Human prostate carcinoma epithelial cell) whole cell lysates
      Lane 2 : Human brain lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 86 kDa


      Exposure time: 30 seconds


      We recommend to use 2% BSA as antibody dilution buffer to get desired WB results

       

      Diluting buffer and concentration:

      Left Lanes: 5% NFDM/TBST
      Right Lanes: 2% BSA/TBST

    References

    This product has been referenced in:

    • Onishi S  et al. Green Tea Extracts Attenuate Brain Dysfunction in High-Fat-Diet-Fed SAMP8 Mice. Nutrients 11:N/A (2019). Read more (PubMed: 30979047) »
    • Flores J  et al. Caspase-1 inhibition alleviates cognitive impairment and neuropathology in an Alzheimer's disease mouse model. Nat Commun 9:3916 (2018). Read more (PubMed: 30254377) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you or your inquiry.
    I can confirm that ab79423 is a rabbit monoclonal antibody of the IgG isotype.
    Unlike mice, rabbits do not have sub-isotypes like IgG2a for example.
    I recommend to choose a isotype control that also is monoclonal and called rabbit IgG monoclonal. The second best option would be polyclonal rabbit IgG.
    A suitable isotype control would be
    ab125938 https://www.abcam.com/index.html?datasheet=125938 https://www.abcam.com/index.html?datasheet=125938.
    I hope this information is helpful and wish you good luck with your research.

    Read More

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