Product nameAnti-CD10 antibody [EPR2997]
See all CD10 primary antibodies
DescriptionRabbit monoclonal [EPR2997] to CD10
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to residues on the N terminal of Human CD10.
- WB: LNCAP, Human brain, Mouse brain, and Rat brain lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab79423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).|
|Flow Cyt||1/100 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similaritiesBelongs to the peptidase M13 family.
Cellular localizationCell membrane.
- Information by UniProt
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
All lanes : Anti-CD10 antibody [EPR2997] (ab79423) at 1/2000 dilution
Lane 1 : LNCAP (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 2 : Human brain lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Exposure time: 30 seconds
We recommend to use 2% BSA as antibody dilution buffer to get desired WB results
Diluting buffer and concentration:
Left Lanes: 5% NFDM/TBST
Right Lanes: 2% BSA/TBST
This product has been referenced in:
- Onishi S et al. Green Tea Extracts Attenuate Brain Dysfunction in High-Fat-Diet-Fed SAMP8 Mice. Nutrients 11:N/A (2019). Read more (PubMed: 30979047) »
- Flores J et al. Caspase-1 inhibition alleviates cognitive impairment and neuropathology in an Alzheimer's disease mouse model. Nat Commun 9:3916 (2018). Read more (PubMed: 30254377) »