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  1. Link

    cd10-antibody-epr5904-110-ab208778.pdf

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Immunology Adaptive Immunity B Cells CD
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD10 antibody [EPR5904-110] (ab208778)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (5)

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Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
  • Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
  • Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)
  • Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)
  • Anti-CD10 antibody [EPR5904-110] (ab208778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5904-110] to CD10
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Alexa Fluor® 488 Anti-CD10 antibody [EPR5904-110] (ab225479)

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Overview

  • Product name

    Anti-CD10 antibody [EPR5904-110]
    See all CD10 primary antibodies
  • Description

    Rabbit monoclonal [EPR5904-110] to CD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LNCaP, Raji and Ramos whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and breast cancer tissues. IP: LNCaP whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5904-110
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers

Associated products

  • Alternative Versions

    • Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)
    • Alexa Fluor® 488 Anti-CD10 antibody [EPR5904-110] (ab225479)
    • Alexa Fluor® 647 Anti-CD10 antibody [EPR5904-110] (ab225480)
    • Anti-CD10 antibody [EPR5904-110] - BSA and Azide free (ab271985)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human CD10 protein (ab157051)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 90-110 kDa (predicted molecular weight: 85 kDa).
IP
1/20.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 90-110 kDa (predicted molecular weight: 85 kDa).
IP
1/20.
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similarities

    Belongs to the peptidase M13 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P08473 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4311 Human
    • Omim: 120520 Human
    • SwissProt: P08473 Human
    • Unigene: 307734 Human
    • Alternative names

      • Atriopeptidase antibody
      • CALLA antibody
      • CD10 antibody
      • CD10 antigen antibody
      • Common acute lymphocytic leukemia antigen antibody
      • DKFZp686O16152 antibody
      • EC 3.4.24.11 antibody
      • Enkephalinase antibody
      • EPN antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
      • Membrane metallo endopeptidase antibody
      • Membrane metallo endopeptidase variant 1 antibody
      • Membrane metallo endopeptidase variant 2 antibody
      • Membrane metalloendopeptidase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
      • Membrane metalloendopeptidase variant 1 antibody
      • Membrane metalloendopeptidase variant 2 antibody
      • MGC126681 antibody
      • MGC126707 antibody
      • MME antibody
      • NEP antibody
      • NEP_HUMAN antibody
      • Neprilysin antibody
      • neprilysin-390 antibody
      • neprilysin-411 antibody
      • Neutral endopeptidase 24.11 antibody
      • Neutral endopeptidase antibody
      • Neutral endopeptidase, membrane-associated antibody
      • SFE antibody
      • Skin fibroblast elastase antibody
      see all

    Images

    • Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: CD10 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: RAJI whole cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab208778 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab208778 was shown to recognize CD10 when CD10 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD10 knockout samples were subjected to SDS-PAGE.  Ab208778 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
      All lanes : Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/1000 dilution

      Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
      Lane 3 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 85 kDa
      Observed band size: 90-110 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/10000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 85 kDa
      Observed band size: 90-110 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)

      Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)
      Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)

      CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.

      Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: LNCaP whole cell lysate, 10µg (Input).

      Lane 2: ab208778 IP in LNCaP whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Anti-CD10 antibody [EPR5904-110] (ab208778)
      Anti-CD10 antibody [EPR5904-110] (ab208778)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (5)

    Publishing research using ab208778? Please let us know so that we can cite the reference in this datasheet.

    ab208778 has been referenced in 5 publications.

    • Suzuki M  et al. Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma. Proteomics Clin Appl 14:e1900091 (2020). PubMed: 31721454
    • Wendel JRH  et al. Three-Dimensional Biofabrication Models of Endometriosis and the Endometriotic Microenvironment. Biomedicines 8:N/A (2020). PubMed: 33233463
    • Feng CZ  et al. Dendrobium polysaccharides attenuate cognitive impairment in senescence-accelerated mouse prone 8 mice via modulation of microglial activation. Brain Res 1704:1-10 (2019). PubMed: 30253123
    • Suda K  et al. Different mutation profiles between epithelium and stroma in endometriosis and normal endometrium. Hum Reprod 34:1899-1905 (2019). PubMed: 31621846
    • Raposo TP  et al. CD10 inhibits cell motility but expression is associated with advanced stage disease in colorectal cancer. Exp Mol Pathol 104:190-198 (2018). IF ; Human . PubMed: 29653092

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