Recombinant Anti-CD10 antibody [EPR5904-110] (ab208778)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-CD10 antibody [EPR5904-110]
See all CD10 primary antibodies -
Description
Rabbit monoclonal [EPR5904-110] to CD10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD10 aa 50-500. The exact sequence is proprietary.
Database link: P08473 -
Positive control
- WB: LNCaP, Raji and Ramos whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and breast cancer tissues. IP: LNCaP whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5904-110 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab208778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 90-110 kDa (predicted molecular weight: 85 kDa). | |
| IP | 1/20. | |
| IHC-P | 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4311 Human
- Omim: 120520 Human
- SwissProt: P08473 Human
- Unigene: 307734 Human
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Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
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![Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)](https://www.abcam.com/ps/products/208/ab208778/Images/ab208778-270788-anti-cd10-antibody-epr5904-110-western-blot.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CD10 knockout HAP1 whole cell lysate (20 µg)
Lane 3: RAJI whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab208778 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.ab208778 was shown to recognize CD10 when CD10 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD10 knockout samples were subjected to SDS-PAGE. Ab208778 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)](https://www.abcam.com/ps/products/208/ab208778/Images/ab208778-254738-anti-cd10-antibody-epr5904-110-western-blot.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)All lanes : Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)](https://www.abcam.com/ps/products/208/ab208778/Images/ab208778-254737-anti-cd10-antibody-epr5904-110-western-blot.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] (ab208778)Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)](https://www.abcam.com/ps/products/208/ab208778/Images/ab208778-254736-anti-cd10-antibody-epr5904-110-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)](https://www.abcam.com/ps/products/208/ab208778/Images/ab208778-254735-anti-cd10-antibody-epr5904-110-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] (ab208778)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)](https://www.abcam.com/ps/products/208/ab208778/Images/ab208778-254734-anti-cd10-antibody-epr5904-110-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] (ab208778)CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.
Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: LNCaP whole cell lysate, 10µg (Input).
Lane 2: ab208778 IP in LNCaP whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Datasheets and documents
References
This product has been referenced in:
- Feng CZ et al. Dendrobium polysaccharides attenuate cognitive impairment in senescence-accelerated mouse prone 8 mice via modulation of microglial activation. Brain Res 1704:1-10 (2019). Read more (PubMed: 30253123) »
- Raposo TP et al. CD10 inhibits cell motility but expression is associated with advanced stage disease in colorectal cancer. Exp Mol Pathol 104:190-198 (2018). IF ; Human . Read more (PubMed: 29653092) »
