Recombinant Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)
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- References
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Overview
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Product name
Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free
See all CD10 primary antibodies -
Description
Rabbit monoclonal [EPR5904-110] to CD10 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment aa 50-500. The exact sequence is proprietary.
Database link: P08473 -
Positive control
- WB: LNCaP, Raji and Ramos whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and breast cancer tissues. IP: LNCaP whole cell lysate.
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General notes
ab222225 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5904-110 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab222225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 90-110 kDa (predicted molecular weight: 85 kDa). |
Target
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4311 Human
- Omim: 120520 Human
- SwissProt: P08473 Human
- Unigene: 307734 Human
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Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-321085-anti-cd10-antibody-epr5904-110-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-321084-anti-cd10-antibody-epr5904-110-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-321083-anti-cd10-antibody-epr5904-110-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.
Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: LNCaP whole cell lysate, 10µg (Input).
Lane 2: ab208778 IP in LNCaP whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Datasheets and documents
References
ab222225 has not yet been referenced specifically in any publications.
