Recombinant Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)
![Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-483328-ab208778254738anticd10antibodyepr5904110westernblot.jpg "Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5904-110] to CD10 - Low endotoxin, Azide free
- Suitable for: IHC-P, IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free
See all CD10 primary antibodies -
Description
Rabbit monoclonal [EPR5904-110] to CD10 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: LNCaP, Raji and Ramos whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and breast cancer tissues. IP: LNCaP whole cell lysate.
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General notes
ab222225 is the carrier-free version of ab208778.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5904-110 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 90-110 kDa (predicted molecular weight: 85 kDa).
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 90-110 kDa (predicted molecular weight: 85 kDa). |
Target
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4311 Human
- Omim: 120520 Human
- SwissProt: P08473 Human
- Unigene: 307734 Human
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Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
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Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)All lanes : Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-483327-ab208778254737anticd10antibodyepr5904110westernblot.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-483326-ab208778270788anticd10antibodyepr5904110westernblot.jpg)
Western blot - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CD10 knockout HAP1 whole cell lysate (20 µg)
Lane 3: RAJI whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab208778 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.ab208778 was shown to recognize CD10 when CD10 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD10 knockout samples were subjected to SDS-PAGE. Ab208778 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-321085-anti-cd10-antibody-epr5904-110-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-321084-anti-cd10-antibody-epr5904-110-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-321083-anti-cd10-antibody-epr5904-110-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.
Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: LNCaP whole cell lysate, 10µg (Input).
Lane 2: ab208778 IP in LNCaP whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
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![Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)](https://www.abcam.com/ps/products/222/ab222225/Images/ab222225-4-benefits-of-recombinant-antibodies.png)
Anti-CD10 antibody [EPR5904-110] - Low endotoxin, Azide free (ab222225)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab222225 has not yet been referenced specifically in any publications.
