Recombinant
RabMAb

Recombinant Anti-CD10 antibody [SP179] - N-terminal (ab227659)

Overview

  • Product name

    Anti-CD10 antibody [SP179] - N-terminal
    See all CD10 primary antibodies
  • Description

    Rabbit monoclonal [SP179] to CD10 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD10 aa 1-100 (N terminal) (phospho S4 + S6). The exact sequence is proprietary.
    Database link: P08473

  • Positive control

    • IHC-P: Human kidney, breast, placenta, liver, prostate, renal cell carcinoma, Mouse kidney, and Rat kidney tissue; WB: LNCaP cell lysate; FC: Ramos, 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab227659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/400.
WB 1/400. Predicted molecular weight: 85 kDa.
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Incubate with primary antibody for 10 minutes at room temperature.

Target

  • Function

    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similarities

    Belongs to the peptidase M13 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atriopeptidase antibody
    • CALLA antibody
    • CD10 antibody
    • CD10 antigen antibody
    • Common acute lymphocytic leukemia antigen antibody
    • DKFZp686O16152 antibody
    • EC 3.4.24.11 antibody
    • Enkephalinase antibody
    • EPN antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
    • Membrane metallo endopeptidase antibody
    • Membrane metallo endopeptidase variant 1 antibody
    • Membrane metallo endopeptidase variant 2 antibody
    • Membrane metalloendopeptidase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
    • Membrane metalloendopeptidase variant 1 antibody
    • Membrane metalloendopeptidase variant 2 antibody
    • MGC126681 antibody
    • MGC126707 antibody
    • MME antibody
    • NEP antibody
    • NEP_HUMAN antibody
    • Neprilysin antibody
    • neprilysin-390 antibody
    • neprilysin-411 antibody
    • Neutral endopeptidase 24.11 antibody
    • Neutral endopeptidase antibody
    • Neutral endopeptidase, membrane-associated antibody
    • SFE antibody
    • Skin fibroblast elastase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-CD10 antibody [SP179] - N-terminal (ab227659) at 1/400 dilution + LNCaP (human prostate cancer cell line) cell lysate

    Predicted band size: 85 kDa

  • Flow cytometry analysis of 2.4G2 (Rat B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Flow cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte ) labeling CD10 with purified ab227659 at 1/200 dilution (0.705 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/20 dilution (7.05 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling CD10 with ab227659 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue). 

  • Formalin-fixed, paraffin-embedded human breast tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

References

ab227659 has not yet been referenced specifically in any publications.

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