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    cd10-antibody-sp67-ab227640.pdf

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Immunology Adaptive Immunity B Cells CD
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RecombinantRabMAb

Recombinant Anti-CD10 antibody [SP67] (ab227640)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)
  • Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)
  • Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)
  • Western blot - Anti-CD10 antibody [SP67] (ab227640)
  • Anti-CD10 antibody [SP67] (ab227640)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP67] to CD10
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-CD10 antibody [SP67]
    See all CD10 primary antibodies
  • Description

    Rabbit monoclonal [SP67] to CD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos cell lysate. IHC-P: Human renal cell carcinoma tissue. Flow Cyt: Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP67
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers

Associated products

  • Alternative Versions

    • Anti-CD10 antibody [SP67] - BSA and Azide free (ab236235)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant human CD10 protein (ab157051)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/100.

Perform heat mediated antigen retrieval with 1mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

WB
1/25. Predicted molecular weight: 86 kDa.

Primary antibody incubation for 1 hour at room temperature.

Flow Cyt
1/200.
Notes
IHC-P
1/100.

Perform heat mediated antigen retrieval with 1mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

WB
1/25. Predicted molecular weight: 86 kDa.

Primary antibody incubation for 1 hour at room temperature.

Flow Cyt
1/200.

Target

  • Function

    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similarities

    Belongs to the peptidase M13 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P08473 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 425031 Chicken
    • Entrez Gene: 536741 Cow
    • Entrez Gene: 4311 Human
    • Entrez Gene: 17380 Mouse
    • Entrez Gene: 24590 Rat
    • Omim: 120520 Human
    • SwissProt: P08473 Human
    • SwissProt: Q61391 Mouse
    • SwissProt: P07861 Rat
    • Unigene: 307734 Human
    • Unigene: 296022 Mouse
    • Unigene: 33598 Rat
    see all
  • Alternative names

    • Atriopeptidase antibody
    • CALLA antibody
    • CD10 antibody
    • CD10 antigen antibody
    • Common acute lymphocytic leukemia antigen antibody
    • DKFZp686O16152 antibody
    • EC 3.4.24.11 antibody
    • Enkephalinase antibody
    • EPN antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
    • Membrane metallo endopeptidase antibody
    • Membrane metallo endopeptidase variant 1 antibody
    • Membrane metallo endopeptidase variant 2 antibody
    • Membrane metalloendopeptidase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
    • Membrane metalloendopeptidase variant 1 antibody
    • Membrane metalloendopeptidase variant 2 antibody
    • MGC126681 antibody
    • MGC126707 antibody
    • MME antibody
    • NEP antibody
    • NEP_HUMAN antibody
    • Neprilysin antibody
    • neprilysin-390 antibody
    • neprilysin-411 antibody
    • Neutral endopeptidase 24.11 antibody
    • Neutral endopeptidase antibody
    • Neutral endopeptidase, membrane-associated antibody
    • SFE antibody
    • Skin fibroblast elastase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human renal cell carcinoma tissue sections labeling CD10 with ab227640 at 1/100 dilution (1.89 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human renal cell carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227640 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227640 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)
    Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)
    Flow Cytometry analysis of Jurkat(Human T cell leukemia T lymphocyte)_negative control cells labeling CD10 with purified ab227640 at 1:200 dilution (1.025 µg/ml) Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)
    Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)
    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CD10 with purified ab227640 at 1:200 dilution (1.025 µg/ml) Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Western blot - Anti-CD10 antibody [SP67] (ab227640)
    Western blot - Anti-CD10 antibody [SP67] (ab227640)
    Anti-CD10 antibody [SP67] (ab227640) at 1/25 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 86 kDa

  • Anti-CD10 antibody [SP67] (ab227640)
    Anti-CD10 antibody [SP67] (ab227640)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab227640? Please let us know so that we can cite the reference in this datasheet.

ab227640 has been referenced in 1 publication.

  • Foster DS  et al. Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nat Commun 11:4061 (2020). PubMed: 32792541

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD10 antibody [SP67]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

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Verified customer

Submitted Sep 17 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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