Recombinant Anti-CD10 antibody [SP67] (ab227640)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP67] to CD10
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD10 antibody [SP67]
See all CD10 primary antibodies -
Description
Rabbit monoclonal [SP67] to CD10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Synthetic peptide within Human CD10 aa 450-550. The exact sequence is proprietary.
Database link: P08473 -
Positive control
- WB: Ramos cell lysate. IHC-P: Human renal cell carcinoma tissue. Flow Cyt: Raji cells.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP67 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with 1mM EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
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| WB | 1/25. Predicted molecular weight: 86 kDa. Primary antibody incubation for 1 hour at room temperature. |
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| Flow Cyt | 1/200. |
Target
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 425031 Chicken
- Entrez Gene: 536741 Cow
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Entrez Gene: 24590 Rat
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
see all -
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-5-AntiCD10-antibody-SP67-IHC-human-renal-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human renal cell carcinoma tissue sections labeling CD10 with ab227640 at 1/100 dilution (1.89 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human renal cell carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227640 for 30 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-290389-anti-cd10-antibody-sp67-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227640 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-338496-anti-cd10-antibody-sp67-flow-cytometry.jpg)
Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)Flow Cytometry analysis of Jurkat(Human T cell leukemia T lymphocyte)_negative control cells labeling CD10 with purified ab227640 at 1:200 dilution (1.025 µg/ml) Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-338495-anti-cd10-antibody-sp67-flow-cytometry.jpg)
Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CD10 with purified ab227640 at 1:200 dilution (1.025 µg/ml) Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Western blot - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-290390-anti-cd10-antibody-sp67-western-blot.jpg)
Western blot - Anti-CD10 antibody [SP67] (ab227640)Anti-CD10 antibody [SP67] (ab227640) at 1/25 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 86 kDa -
![Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-6-benefits-of-recombinant-antibodies.png)
Anti-CD10 antibody [SP67] (ab227640)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab227640 has not yet been referenced specifically in any publications.
