Recombinant Anti-CD10 antibody [SP67] (ab227640)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-5-AntiCD10-antibody-SP67-IHC-human-renal-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP67] to CD10
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD10 antibody [SP67]
See all CD10 primary antibodies -
Description
Rabbit monoclonal [SP67] to CD10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos cell lysate. IHC-P: Human renal cell carcinoma tissue. Flow Cyt: Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP67 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/100.
Perform heat mediated antigen retrieval with 1mM EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
| WB |
1/25. Predicted molecular weight: 86 kDa.
Primary antibody incubation for 1 hour at room temperature. |
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| Flow Cyt |
1/200.
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| Notes |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with 1mM EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
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WB
1/25. Predicted molecular weight: 86 kDa. Primary antibody incubation for 1 hour at room temperature. |
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Flow Cyt
1/200. |
Target
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 425031 Chicken
- Entrez Gene: 536741 Cow
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Entrez Gene: 24590 Rat
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
see all -
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human renal cell carcinoma tissue sections labeling CD10 with ab227640 at 1/100 dilution (1.89 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human renal cell carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227640 for 30 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-290389-anti-cd10-antibody-sp67-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP67] (ab227640)Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227640 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-338496-anti-cd10-antibody-sp67-flow-cytometry.jpg)
Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)Flow Cytometry analysis of Jurkat(Human T cell leukemia T lymphocyte)_negative control cells labeling CD10 with purified ab227640 at 1:200 dilution (1.025 µg/ml) Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-338495-anti-cd10-antibody-sp67-flow-cytometry.jpg)
Flow Cytometry - Anti-CD10 antibody [SP67] (ab227640)Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CD10 with purified ab227640 at 1:200 dilution (1.025 µg/ml) Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Western blot - Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-290390-anti-cd10-antibody-sp67-western-blot.jpg)
Western blot - Anti-CD10 antibody [SP67] (ab227640)Anti-CD10 antibody [SP67] (ab227640) at 1/25 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 86 kDa -
![Anti-CD10 antibody [SP67] (ab227640)](https://www.abcam.com/ps/products/227/ab227640/Images/ab227640-6-benefits-of-recombinant-antibodies.png)
Anti-CD10 antibody [SP67] (ab227640)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227640 has been referenced in 1 publication.
- Foster DS et al. Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nat Commun 11:4061 (2020). PubMed: 32792541