Recombinant Anti-CD103 antibody [EPR22590-27] (ab224202)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-9-224202IHC4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22590-27] to CD103
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD103 antibody [EPR22590-27]
See all CD103 primary antibodies -
Description
Rabbit monoclonal [EPR22590-27] to CD103 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human CD103 aa 650-1000. The exact sequence is proprietary.
Database link: P38570 -
Positive control
- IHC-P: Rat and human colon tissue. Mouse spleen tissue. Human lung carcinoma tissue. IHC-Fr: Human and mouse colon tissue. WB: Mouse and rat stomach, thymus and spleen cell lysate. His-tagged human CD103 recombinant protein fragment (aa676-965).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22590-27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Immunohistochemistry reagents
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab224202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa). | |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. 1/5000 for mouse and rat samples. 1/1000 for human samples. |
|
| IHC-Fr | 1/100. |
Target
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Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. -
Tissue specificity
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3682 Human
- Entrez Gene: 16407 Mouse
- Entrez Gene: 83577 Rat
- Omim: 604682 Human
- SwissProt: P38570 Human
- SwissProt: Q60677 Mouse
- Unigene: 513867 Human
- Unigene: 96 Mouse
see all -
Alternative names
- A530055J10 antibody
- alpha-E1 antibody
- alpha-M290 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CD103 with ab224202 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the lymphocytes in the rat colon (PMID: 23355207) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-8-224202IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD103 with ab224202 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the lymphocytes in the mouse spleen (PMID: 23355207) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-7-224202IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling CD103 with ab224202 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphocytes in the human lung carcinoma (PMID: 25725111). The section was incubated with ab224202 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-6-224202IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD103 with ab224202 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphocytes in the human colon (PMID: 16775489, 30405182, 23355207). The section was incubated with ab224202 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-5-224202IHCFr2.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling CD103 with ab224202 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the lymphocytes in the rat colon (PMID: 16775489) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). -
![Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-4-224202IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling CD103 with ab224202 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the lymphocytes in the mouse colon (PMID: 16775489) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). -
![Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-344173-AntiCD103-antibody-EPR2259027-westen-blot-mouse-rat-stomach-spleen-thymus.jpg)
Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)All lanes : Anti-CD103 antibody [EPR22590-27] (ab224202) at 1/1000 dilution
Lane 1 : Mouse stomach cell lysate
Lane 2 : Mouse spleen cell lysate
Lane 3 : Mouse thymus cell lysate
Lane 4 : Rat stomach cell lysate
Lane 5 : Rat spleen cell lysate
Lane 6 : Rat thymus cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:26921343; PMID:29021139).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-2-224202WB2.jpg)
Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)Anti-CD103 antibody [EPR22590-27] (ab224202) at 1/1000 dilution + His-tagged human CD103 recombinant protein fragment (aa676-965), 20ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 130 kDa
Exposure time: 6 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-11-benefits-of-recombinant-antibodies.png)
Anti-CD103 antibody [EPR22590-27] (ab224202)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab224202 has not yet been referenced specifically in any publications.
