Recombinant Anti-CD103 antibody [EPR22590-27] (ab224202)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD103 antibody [EPR22590-27]
See all CD103 primary antibodies -
Description
Rabbit monoclonal [EPR22590-27] to CD103 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human CD103 aa 650-1000. The exact sequence is proprietary.
Database link: P38570 -
Positive control
- IHC-P: Rat and human colon tissue. Mouse spleen tissue. Human lung carcinoma tissue. IHC-Fr: Human and mouse colon tissue. WB: Mouse and rat stomach, thymus and spleen cell lysate. His-tagged human CD103 recombinant protein fragment (aa676-965).
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22590-27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Immunohistochemistry reagents
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab224202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa). | |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. 1/5000 for mouse and rat samples. 1/1000 for human samples. |
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| IHC-Fr | 1/100. |
Target
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Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. -
Tissue specificity
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3682 Human
- Entrez Gene: 16407 Mouse
- Entrez Gene: 83577 Rat
- Omim: 604682 Human
- SwissProt: P38570 Human
- SwissProt: Q60677 Mouse
- Unigene: 513867 Human
- Unigene: 96 Mouse
see all -
Alternative names
- A530055J10 antibody
- alpha-E1 antibody
- alpha-M290 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-9-224202IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CD103 with ab224202 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the lymphocytes in the rat colon (PMID: 23355207) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-8-224202IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD103 with ab224202 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the lymphocytes in the mouse spleen (PMID: 23355207) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-7-224202IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling CD103 with ab224202 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphocytes in the human lung carcinoma (PMID: 25725111). The section was incubated with ab224202 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-6-224202IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD103 with ab224202 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphocytes in the human colon (PMID: 16775489, 30405182, 23355207). The section was incubated with ab224202 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-5-224202IHCFr2.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling CD103 with ab224202 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the lymphocytes in the rat colon (PMID: 16775489) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). -
![Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-4-224202IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] (ab224202)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling CD103 with ab224202 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the lymphocytes in the mouse colon (PMID: 16775489) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). -
![Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-344173-AntiCD103-antibody-EPR2259027-westen-blot-mouse-rat-stomach-spleen-thymus.jpg)
Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)All lanes : Anti-CD103 antibody [EPR22590-27] (ab224202) at 1/1000 dilution
Lane 1 : Mouse stomach cell lysate
Lane 2 : Mouse spleen cell lysate
Lane 3 : Mouse thymus cell lysate
Lane 4 : Rat stomach cell lysate
Lane 5 : Rat spleen cell lysate
Lane 6 : Rat thymus cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:26921343; PMID:29021139).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)](https://www.abcam.com/ps/products/224/ab224202/Images/ab224202-2-224202WB2.jpg)
Western blot - Anti-CD103 antibody [EPR22590-27] (ab224202)Anti-CD103 antibody [EPR22590-27] (ab224202) at 1/1000 dilution + His-tagged human CD103 recombinant protein fragment (aa676-965), 20ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 130 kDa
Exposure time: 6 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab224202 has not yet been referenced specifically in any publications.
