Recombinant Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-6-224202IHC4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22590-27] to CD103 - BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD103 antibody [EPR22590-27] - BSA and Azide free
See all CD103 primary antibodies -
Description
Rabbit monoclonal [EPR22590-27] to CD103 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat and human colon tissue. Mouse spleen tissue. Human lung carcinoma tissue. IHC-Fr: Human and mouse colon tissue.
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General notes
ab254201 is the carrier-free version of ab224202.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22590-27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Immunohistochemistry reagents
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. -
Tissue specificity
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3682 Human
- Entrez Gene: 16407 Mouse
- Entrez Gene: 83577 Rat
- Omim: 604682 Human
- SwissProt: P38570 Human
- SwissProt: Q60677 Mouse
- Unigene: 513867 Human
- Unigene: 96 Mouse
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Alternative names
- A530055J10 antibody
- alpha-E1 antibody
- alpha-M290 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CD103 with ab224202 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the lymphocytes in the rat colon (PMID: 23355207) is observed. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224202).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-5-224202IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD103 with ab224202 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the lymphocytes in the mouse spleen (PMID: 23355207) is observed. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224202).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-4-224202IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling CD103 with ab224202 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphocytes in the human lung carcinoma (PMID: 25725111). The section was incubated with ab224202 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224202).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-3-224202IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD103 with ab224202 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the lymphocytes in the human colon (PMID: 16775489, 30405182, 23355207). The section was incubated with ab224202 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224202).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
![Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-2-224202IHCFr2.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling CD103 with ab224202 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the lymphocytes in the rat colon (PMID: 16775489) is observed. Counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224202).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). -
![Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-1-224202IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling CD103 with ab224202 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the lymphocytes in the mouse colon (PMID: 16775489) is observed. Counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224202).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). -
![Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)](https://www.abcam.com/ps/products/254/ab254201/Images/ab254201-7-benefits-of-recombinant-antibodies.png)
Anti-CD103 antibody [EPR22590-27] - BSA and Azide free (ab254201)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab254201 has been referenced in 1 publication.
- Schwabenland M et al. Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions. Immunity 54:1594-1610.e11 (2021). PubMed: 34174183
