Recombinant
RabMAb

Recombinant Anti-CD103 antibody [EPR4166(2)] (ab129202)

Rabbit recombinant monoclonal CD103 antibody [EPR4166(2)]. Validated in IHC and tested in Human. Cited in 10 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-CD103 antibody [EPR4166(2)]
    See all CD103 primary antibodies
  • Description

    Rabbit monoclonal [EPR4166(2)] to CD103
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD103 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: P38570

  • Positive control

    • IHC-P: Human colon, tonsil, stomach and ileum tissues.
  • General notes

    A trial size is available to purchase for this antibody.

    Clone EPR4166(2) is different from clone EPR4166.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
  • Tissue specificity

    Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A530055J10 antibody
    • alpha-E1 antibody
    • alpha-M290 antibody
    • aM290 antibody
    • Antigen CD103 antibody
    • CD 103 antibody
    • CD103 antibody
    • CD103 antigen antibody
    • HML 1 antigen antibody
    • HML-1 antigen antibody
    • HML1 antigen antibody
    • Human mucosal lymphocyte antigen 1 antibody
    • Human mucosal lymphocyte antigen 1, alpha subunit antibody
    • Huminae antibody
    • Integrin alpha E antibody
    • Integrin alpha E heavy chain antibody
    • Integrin alpha E light chain antibody
    • Integrin alpha E, epithelial-associated antibody
    • Integrin alpha E1 antibody
    • Integrin alpha IEL antibody
    • Integrin alpha M290 antibody
    • Integrin alpha-E heavy chain antibody
    • Integrin alpha-IEL antibody
    • Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
    • Integrin, alpha E antibody
    • ITAE_HUMAN antibody
    • Itgae antibody
    • MGC141996 antibody
    • MGC183601 antibody
    • Mucosal lymphocyte 1 antigen antibody
    • Mucosal lymphocyte antigen 1 antibody
    see all

Images

  • Anti-CD103/Integrin alpha E antibody [EPR4166(2)] (ab129202)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD103/Integrin alpha E with ab129202 at a dilution of 1:1000. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
    This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with purified ab129202 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody (inset).

    Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with unpurified ab129202 at a dilution of 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling CD103 with unpurified ab129202.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human ileum tissue labeling CD103 with unpurified ab129202.

  • Unpurified ab129202 showing positive staining or CD103 in normal stomach tissue.

  • Unpurified ab129202 showing negative staining in colonic adenocarcinoma tissue.

  • Unpurified ab129202 showing negative staining in normal uterus tissue.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Shields BD  et al. Loss of E-Cadherin Inhibits CD103 Antitumor Activity and Reduces Checkpoint Blockade Responsiveness in Melanoma. Cancer Res 79:1113-1123 (2019). Read more (PubMed: 30674537) »
  • Mann JE  et al. Analysis of tumor-infiltrating CD103 resident memory T-cell content in recurrent laryngeal squamous cell carcinoma. Cancer Immunol Immunother 68:213-220 (2019). Read more (PubMed: 30361882) »
See all 13 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Duodenum)
Permeabilization
No
Specification
Duodenum
Blocking step
V-blocker as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde

Dr. Li Li

Verified customer

Submitted Aug 01 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Tonsil
Blocking step
Bloxall Endogenous Peroxidase and Alkaline Phosphatase Blocking as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Mr. Joshua Dodge

Verified customer

Submitted Jan 22 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Ovarian Tumor)
Permeabilization
Yes - PBS + 0.3% Triton X-100
Specification
Ovarian Tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (doudenum celiac disease with intraepithelial lymph)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 min
Permeabilization
No
Specification
doudenum celiac disease with intraepithelial lymph
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary tumor (MMATV-PyMT))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 5 min high pressure
Permeabilization
Yes - DAKO TRS
Specification
Mammary tumor (MMATV-PyMT)
Blocking step
5% goat serum +3% BSA in IF buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 14 2015

Answer

Thank you for your inquiry.

We currently have two vials in stock. They both have a concentration of 0.948 mg/ml.

Icannot confirmthe concentration of future lots.

I also would like to comment on the concentration:

The IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas.

Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different.

Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction.

I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube.

I hope this information is helpful. Please do not hesitate to contact me with any further questions in this matter.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Tonsil-cynomolgus monkey)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0
Permeabilization
No
Specification
Tonsil-cynomolgus monkey
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
Fixative
Formaldehyde

Jing Ma

Verified customer

Submitted Jul 30 2012

Question
Answer

DISCOUNT CODE: *
Expiration date:*

I am very pleased to hear you would like to accept our offer and test ab129202 in Cyno Monkey. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Cyno Monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam.

Under our Abpromise we do guarantee that ab129202 will work in IHC-P on human, mouse and rat tissue and that it is specific to Integrin alpha E (also known as CD103). I looked up the antibody on the Epitomics website and they have the same application and species reactivity as listed on the Abcam website:




If there is anything else I can help you with, please let me know.

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