Overview

  • Product name

    Anti-CD103 antibody [SP301]
    See all CD103 primary antibodies
  • Description

    Rabbit monoclonal [SP301] to CD103
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD103 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P38570

  • Positive control

    • IHC-P: Human tonsil, stomach carcinoma, stomach, colon carcinoma, colon, breast, lung squamous cell carcinoma, thymus, cervix, skin squamous cell carcinoma, and Human cervical carcinoma tissue; FC: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate for 10 minutes at room temperature.

Flow Cyt 1/100.

Incubate with primary antibody for 30 minutes at 4 °C.

Target

  • Function

    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
  • Tissue specificity

    Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A530055J10 antibody
    • alpha-E1 antibody
    • alpha-M290 antibody
    • aM290 antibody
    • Antigen CD103 antibody
    • CD 103 antibody
    • CD103 antibody
    • CD103 antigen antibody
    • HML 1 antigen antibody
    • HML-1 antigen antibody
    • HML1 antigen antibody
    • Human mucosal lymphocyte antigen 1 antibody
    • Human mucosal lymphocyte antigen 1, alpha subunit antibody
    • Huminae antibody
    • Integrin alpha E antibody
    • Integrin alpha E heavy chain antibody
    • Integrin alpha E light chain antibody
    • Integrin alpha E, epithelial-associated antibody
    • Integrin alpha E1 antibody
    • Integrin alpha IEL antibody
    • Integrin alpha M290 antibody
    • Integrin alpha-E heavy chain antibody
    • Integrin alpha-IEL antibody
    • Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
    • Integrin, alpha E antibody
    • ITAE_HUMAN antibody
    • Itgae antibody
    • MGC141996 antibody
    • MGC183601 antibody
    • Mucosal lymphocyte 1 antigen antibody
    • Mucosal lymphocyte antigen 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow cytometric analysis of Jurkat (human breast adenocarcinoma cell line) cells labeling CD103 with ab227697 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervix tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

References

ab227697 has not yet been referenced specifically in any publications.

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