• Product name
    Anti-CD105 antibody [3A9]
    See all CD105 primary antibodies
  • Description
    Mouse monoclonal [3A9] to CD105
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human CD105, expressed in E. coli.

  • Positive control
    • IHC-P: Human kidney cancer and stomach cancer tissues ICC/IF: HepG2 cells WB: CD105-hIgGFc transfected HEK293 cell lysate
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR156609-14 are from Tissue Culture Supernatant



Our Abpromise guarantee covers the use of ab114052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 71 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA 1/10000.


  • Function
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all


  • ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human kidney cancer tissue by Immunohistochemistry, with DAB staining.
  • ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human stomach cancer tissue by Immunohistochemistry, with DAB staining.
  • All lanes : Anti-CD105 antibody [3A9] (ab114052) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : CD105-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 71 kDa

  • ab114052, at a 1/200 dilution, staining CD105 in HepG2 cells by Immunofluorescence (green).
    Blue: DRAQ5 fluorescent DNA dye.
    Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of CD105 in HepG2 cells, using ab114052 at a 1/200 dilution (green) and negative control (purple).
  • ELISA using ab114052.


This product has been referenced in:
  • González S  et al. A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16:358-64 (2016). Read more (PubMed: 26896856) »
  • Faber A  et al. Interaction of a CD44+ head and neck squamous cell carcinoma cell line with a stromal cell-derived factor-1-expressing supportive niche: An in vitro model. Oncol Lett 7:82-86 (2014). ICC/IF . Read more (PubMed: 24348826) »

See all 4 Publications for this product

Customer reviews and Q&As

The immunogen used to create this product spans from aa331-567 of human Endoglin (CD105, SwissProt: P17813 ). A BLAST of this sequence shows a 70% possible homology. This is well below our threshold for predicting cross reactivity however as we have no...

Read More

Thank you for contacting us.

Because this antibody has not been purified and is an ascites, the concentration has not been determined. However we estimate that the concentration will be around 5-10 mg/ml.

I hope this information i...

Read More

Thank you for contacting Abcam.

We do have some information that suggests, heat mediated antigen retrieval using citrate buffer would be successful for using ab114052 in IHC-P.

Below is a link some citrate buffer that would be useful ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C

Dr. R Berahovich

Verified customer

Submitted May 24 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More


Sign up