Anti-CD105 antibody [3A9] (ab114052)
Key features and details
- Mouse monoclonal [3A9] to CD105
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD105 antibody [3A9]
See all CD105 primary antibodies -
Description
Mouse monoclonal [3A9] to CD105 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment of Human CD105, expressed in E. coli.
-
Positive control
- IHC-P: Human kidney cancer and stomach cancer tissues ICC/IF: HepG2 cells WB: CD105-hIgGFc transfected HEK293 cell lysate
-
General notes
This product was changed from ascites to supernatant. Lot no’s high than GR156609-14 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A9 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 71 kDa.
|
|
IHC-P | (1) |
1/200 - 1/1000.
|
ICC/IF |
1/200 - 1/1000.
|
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ELISA |
1/10000.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 71 kDa. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ELISA
1/10000. |
Target
-
Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
-
Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
-
Flow cytometric analysis of CD105 in HepG2 cells, using ab114052 at a 1/200 dilution (green) and negative control (purple).
-
ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human kidney cancer tissue by Immunohistochemistry, with DAB staining.
-
ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human stomach cancer tissue by Immunohistochemistry, with DAB staining.
-
All lanes : Anti-CD105 antibody [3A9] (ab114052) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : CD105-hIgGFc transfected HEK293 cell lysate
Predicted band size: 71 kDa -
ab114052, at a 1/200 dilution, staining CD105 in HepG2 cells by Immunofluorescence (green).
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
ELISA using ab114052.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab114052 has been referenced in 15 publications.
- De Filippis R et al. Use of High-Plex Data Reveals Novel Insights into the Tumour Microenvironment of Clear Cell Renal Cell Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 36358805
- Wang X et al. Coaxially Bioprinted Cell-Laden Tubular-Like Structure for Studying Glioma Angiogenesis. Front Bioeng Biotechnol 9:761861 (2021). PubMed: 34660561
- Jain N et al. Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis. Nat Commun 11:3062 (2020). PubMed: 32546788
- Placencio-Hickok VR et al. Soluble CD105 is prognostic of disease recurrence in prostate cancer patients. Endocr Relat Cancer 27:1-9 (2020). PubMed: 31648185
- Ollauri-Ibáñez C et al. Continuous endoglin (CD105) overexpression disrupts angiogenesis and facilitates tumor cell metastasis. Angiogenesis 23:231-247 (2020). PubMed: 31897911