Anti-CD105 antibody (ab137389)
Key features and details
- Rabbit polyclonal to CD105
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD105 antibody
See all CD105 primary antibodies -
Description
Rabbit polyclonal to CD105 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 309-606 of Human CD105 (Uniprot ID: P17813).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; Human SCC15 xenograft tissue. WB: HUVEC Whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 95 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
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Notes |
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WB
1/1000. Detects a band of approximately 95 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Anti-CD105 antibody (ab137389) at 1/1000 dilution + HUVEC whole cell extract at 30 µg
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 antibody (ab137389) diluted at 1:1000.
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Immunohistochemical analysis of paraffin-embedded Human SCC15 xenograft tissue, labelling CD105 with ab137389 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137389 has been referenced in 4 publications.
- Burghardt I et al. Endoglin and TGF-ß signaling in glioblastoma. Cell Tissue Res 384:613-624 (2021). PubMed: 33471197
- Zhang X et al. Bevacizumab promotes active biological behaviors of human umbilical vein endothelial cells by activating TGFß1 pathways via off-VEGF signaling. Cancer Biol Med 17:418-432 (2020). PubMed: 32587778
- Varas-Godoy M et al. Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia. Stem Cells Int 2019:1916542 (2019). PubMed: 30809262
- Krishnan S et al. Modulation of cerebral endothelial cell function by TGF-ß in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition. Oncotarget 6:22480-95 (2015). WB . PubMed: 26090865