Overview

  • Product name
  • Description
    Rabbit polyclonal to CD105
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Sandwich ELISA, ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure recombinant human soluble CD105 (aas 26-586) bearing C-terminal His 6 tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab21224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-226] to CD105 (ab2529). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [MEM-226] to CD105 (ab2529) as Capture.
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Images

  • Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.
  • Flow Cytometry analysis of CD105 in human endothelial precursor cells using ab21224 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.
    Red line= ab21224
    Black line= isotype control

    See Abreview

References

This product has been referenced in:
  • Jackson AM  et al. Endothelial cell antibodies associated with novel targets and increased rejection. J Am Soc Nephrol 26:1161-71 (2015). Read more (PubMed: 25381426) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (endothelial precursor cell)
Specification
endothelial precursor cell
Fixation
none
Permeabilization
No
Gating Strategy
CD133 and CD202B

Dr. A Jackson

Verified customer

Submitted Dec 05 2011

Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab21224 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. After looking at the protocols you used, I am surprised that even using 50ug of protein did not manage to detect any bands. However, it is very hard for me to determine the cause without more details of your WB protocol. If you would be so kind to clarify the following items, it will certainly help me better understand the root of the cause and as a result, I will be able to tag this product as faulty. - did you used a lysis buffer? Was it as strong as RIPA buffer? Please try RIPA buffer as it is the strongest and recommend for extracting membrane bound proteins. - could the no signal you are experiencing be due to poor transfer of protein to membrane? Did you check the transfer efficiency? This can be simply done with a reversible stain such as Ponceau S. - At Abcam, almost all of our products has been tested with 5% BSA. Sometimes, a different blocking buffer might cause cross reaction between the blocking agent and antibodies, therefore causing no bands to appear. I would recommend you try 5% BSA, if you have not already done so. - can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? What was the dilution you used? - was the incubation of the primary antibody done overnight at 4oC? - what type of membrane did you used? - can you confirm the washing buffer used? Was the washing done 15min x 3 times? This is because over washing will eventually cause you to lose signals. I am sorry for the string of questions but I hope the above recommendations may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (contact information, shipping address/purchasing agent, etc.). The purchase number you gave was not found in our system. It should be a 6 digit number. Also, please advice me on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund for you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up