• Product name

    Anti-CD105 antibody [EPR10145-10]
    See all CD105 primary antibodies
  • Description

    Rabbit monoclonal [EPR10145-10] to CD105
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD105 aa 1-200. The exact sequence is proprietary.
    Database link: P17813

  • Positive control

    • HUVEC and ECV-304 cell lysates; Human cervical carcinoma and placenta tissues.
  • General notes

    According to our internal WB data, ab170943 does not react with mouse and rat.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab170943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
    • Tissue specificity

      Endoglin is restricted to endothelial cells in all tissues except bone marrow.
    • Involvement in disease

      Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all


    • All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : CD105 knockout HeLa whole cell lysate
      Lane 3 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 70 kDa

      Lanes 1 - 3: Merged signal (red and green). Green - ab170943 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab170943 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105  knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab170943 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab170943 showing +ve staining in Human lung carcinoma-vessels tissue.

    • ab170943 showing +ve staining in Human normal tonsil-vessels tissue.

    • ab170943 showing +ve staining in Human urinary bladder transitional carcinoma, vessels tissue.

    • ab170943 showing +ve staining in Human normal uterus, vessels tissue.

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD105 with ab170943  at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD105 with ab170943 at 1/250 dilution.

    • All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : ECV-304 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa


    This product has been referenced in:

    • Bychkov A  et al. PSMA expression by microvasculature of thyroid tumors - Potential implications for PSMA theranostics. Sci Rep 7:5202 (2017). Read more (PubMed: 28701709) »
    See 1 Publication for this product

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