Overview

  • Product name

    Anti-CD105 antibody [EPR10145-12]
    See all CD105 primary antibodies
  • Description

    Rabbit monoclonal [EPR10145-12] to CD105
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD105 aa 1-200.
    Database link: P17813

  • Positive control

    • WB: ECV-304 and HUVEC cell lysates, human tonsil tissue lysate and immunoprecipitation pellet from ECV-304 cell lysate. IHC-P: Human glioma, clear cell carcinoma, tonsil and kidney tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 70 kDa.

For unpurified use at 1/50.

IHC-P 1/900.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S endoglin antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Images

  • All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : CD105  knockout HeLa whole cell lysate
    Lane 3 : HUVEC whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 70 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab169545 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab169545 was shown to recognize CD105  in wild-type HeLa cells as signal was lost at the expected MW in CD105  knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105  knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab169545 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with unpurified ab169545 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified)

    Lane 1 : ECV-304 cell lysate
    Lane 2 : Human tonsil cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 70 kDa

  • Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + ECV-304 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + ECV-304 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified) + immunoprecipitation pellet from ECV-304 cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 70 kDa

  • Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + HUVEC cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + HUVEC cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with purified ab169545 at 1/900. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human clear cell carcinoma tissue labelling CD105 with unpurified ab169545 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling CD105 with unpurified ab169545 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD105 with unpurified ab169545 at 1/250.

References

This product has been referenced in:

  • Tang W  et al. The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer. Nat Commun 10:663 (2019). Read more (PubMed: 30737378) »
  • Fang M  et al. The heterogenic tumor microenvironment of hepatocellular carcinoma and prognostic analysis based on tumor neo-vessels, macrophages and a-SMA. Oncol Lett 15:4805-4812 (2018). Read more (PubMed: 29552120) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Normal Human Keratinocyte)
Specification
Normal Human Keratinocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 30 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval
Sample
Cynomolgus Monkey Tissue sections (Bone marrow)
Specification
Bone marrow
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 26 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6
Sample
Human Tissue sections (breast)
Specification
breast
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 22 2013

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