Recombinant Anti-CD105 antibody [EPR19911-220] (ab252345)
![Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-345777-anticd105-antibody-epr19911220-westernblot-human.jpg "Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19911-220] to CD105
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-CD105 antibody [EPR19911-220]
See all CD105 primary antibodies -
Description
Rabbit monoclonal [EPR19911-220] to CD105 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HUVEC whole cell lysates; Rat spleen lysate. IP: HeLa whole cell lysate. IHC-P: Human liver and kidney tissue; rat kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19911-220 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 95, 190 kDa (predicted molecular weight: 71 kDa).
|
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| IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/20.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 95, 190 kDa (predicted molecular weight: 71 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/20. |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2022 Human
- Entrez Gene: 497010 Rat
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)All lanes : Anti-CD105 antibody [EPR19911-220] (ab252345) at 1 µg/ml
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : ENG knockout HeLa whole cell lysate
Lane 3 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 71 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab252345 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab252345 was shown to recognize ENG (Endoglin) in wild-type HeLa cells as signal was lost at the expected MW in ENG knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ENG knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab252345 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-5-252345IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on peritubular microvasculature of rat kidney (PMID: 25381426) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-4-252345IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on sinusoidal endothelial cells of human liver (PMID: 30563158) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-3-252345IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on glomerular and peritubular microvasculature of human kidney (PMID: 25381426) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-2-252345IP1.jpg)
Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] (ab252345)CD105 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab252345 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab252345 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab252345 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252345 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-1-252345WB1.jpg)
Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)All lanes : Anti-CD105 antibody [EPR19911-220] (ab252345) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 71 kDa
Observed band size: 190,95 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 26 seconds.
Lane 2: 6 seconds.
Lane 3: 3 minutes.
The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID: 12746487; 9872992).
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![Anti-CD105 antibody [EPR19911-220] (ab252345)](https://www.abcam.com/ps/products/252/ab252345/Images/ab252345-7-benefits-of-recombinant-antibodies.png)
Anti-CD105 antibody [EPR19911-220] (ab252345)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab252345 has not yet been referenced specifically in any publications.