For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cd105-antibody-epr19911-220-ab252345.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Endothelial Cells
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD105 antibody [EPR19911-220] (ab252345)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)
  • Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] (ab252345)
  • Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)
  • Anti-CD105 antibody [EPR19911-220] (ab252345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19911-220] to CD105
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Rat, Human

You may also be interested in

Primary
Product image
Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

View more associated products

Overview

  • Product name

    Anti-CD105 antibody [EPR19911-220]
    See all CD105 primary antibodies
  • Description

    Rabbit monoclonal [EPR19911-220] to CD105
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HUVEC whole cell lysates; Rat spleen lysate. IP: HeLa whole cell lysate. IHC-P: Human liver and kidney tissue; rat kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19911-220
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry kits

    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human CD105 protein (ab54338)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 95, 190 kDa (predicted molecular weight: 71 kDa).
IHC-P (2)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/20.
Notes
WB
1/1000. Detects a band of approximately 95, 190 kDa (predicted molecular weight: 71 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/20.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
    • Tissue specificity

      Endoglin is restricted to endothelial cells in all tissues except bone marrow.
    • Involvement in disease

      Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P17813 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2022 Human
      • Entrez Gene: 497010 Rat
      • Omim: 131195 Human
      • SwissProt: P17813 Human
      • Unigene: 76753 Human
      • Alternative names

        • AI528660 antibody
        • AI662476 antibody
        • CD 105 antibody
        • CD105 antibody
        • CD105 antigen antibody
        • EGLN_HUMAN antibody
        • END antibody
        • Endoglin antibody
        • Eng antibody
        • FLJ41744 antibody
        • HHT1 antibody
        • ORW antibody
        • ORW1 antibody
        • Osler Rendu Weber syndrome 1 antibody
        • RP11 228B15.2 antibody
        • S endoglin antibody
        • S-endoglin antibody
        • SN6 antibody
        see all

      Images

      • Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)
        Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)
        All lanes : Anti-CD105 antibody [EPR19911-220] (ab252345) at 1 µg/ml

        Lane 1 : Wild-type HeLa whole cell lysate
        Lane 2 : ENG knockout HeLa whole cell lysate
        Lane 3 : HUVEC whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Predicted band size: 71 kDa
        Observed band size: 95 kDa why is the actual band size different from the predicted?



        Lanes 1 - 4: Merged signal (red and green). Green - ab252345 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab252345 was shown to recognize ENG (Endoglin) in wild-type HeLa cells as signal was lost at the expected MW in ENG knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ENG knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab252345 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)

        Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on peritubular microvasculature of rat kidney (PMID: 25381426) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

        The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)

        Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on sinusoidal endothelial cells of human liver (PMID: 30563158) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

        The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] (ab252345)

        Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on glomerular and peritubular microvasculature of human kidney (PMID: 25381426) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

        The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

         

      • Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] (ab252345)
        Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] (ab252345)

        CD105 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab252345 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab252345 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

        Lane 1: HeLa whole cell lysate 10 μg (Input).
        Lane 2: ab252345 IP in HeLa whole cell lysate.
        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252345 in HeLa whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.
        Exposure time: 3 minutes.

      • Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)
        Western blot - Anti-CD105 antibody [EPR19911-220] (ab252345)
        All lanes : Anti-CD105 antibody [EPR19911-220] (ab252345) at 1/1000 dilution

        Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
        Lane 2 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
        Lane 3 : Rat spleen lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 71 kDa
        Observed band size: 190,95 kDa why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure times.

        Lane 1: 26 seconds.

        Lane 2: 6 seconds.

        Lane 3: 3 minutes.

        The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID: 12746487; 9872992).

      • Anti-CD105 antibody [EPR19911-220] (ab252345)
        Anti-CD105 antibody [EPR19911-220] (ab252345)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (0)

      Publishing research using ab252345? Please let us know so that we can cite the reference in this datasheet.

      ab252345 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

      Filter by Species

      Filter by Ratings

      1-2 of 2 Abreviews

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD105 antibody [EPR19911-220]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Metastatic melanoma)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA
      Permeabilization
      Yes - .1% TritonX-100 in PBS
      Specification
      Metastatic melanoma
      Blocking step
      Donkey Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 05 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD105 antibody [EPR19911-220]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Ligament)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris EDTA pH-9
      Permeabilization
      No
      Specification
      Ligament
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 18 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.