Recombinant
RabMAb

Recombinant Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

Overview

  • Product name

    Anti-CD105 antibody [EPR21846] - BSA and Azide free
    See all CD105 primary antibodies
  • Description

    Rabbit monoclonal [EPR21846] to CD105 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CD105 aa 1-600. The exact sequence is proprietary.
    Database link: Q63961

  • Positive control

    • IHC-P: Mouse E14.5 liver tissue.
  • General notes

    Ab231832 is the carrier-free version of ab221675. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab231832 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab231832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681).

WB Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S endoglin antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Images

  • Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line (left panel) and bEND.3 (mouse brain endothelioma cell line) cell line (right panel) labeling CD105 with ab221675 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells.

    Negative control: NIH/3T3. (PMID: 8194490).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution. Positive staining in the endothelial cells of blood vessels in mouse brain tissue section (PMID: 24699047). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Positive staining in the hepatic sinusoidal endothelial cells on mouse liver tissue section (PMID: 12947156; 24507660). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • CD105 was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab221675 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221675 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: bEND.3 whole cell lysate 10 µg (Input). 

    Lane 2: ab221675 IP in bEND.3 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221675 in bEND.3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse lung is observed (PMID: 14528280). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD105 with ab221675 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Neuro-2a cell line. Negative control: NIH/3T3 (PMID: 8194490).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse E14.5 liver is observed (PMID: 18805961). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab231832 has not yet been referenced specifically in any publications.

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