For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cd105-antibody-mem-229-ab69772.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Endothelial Cells
Share by email

Anti-CD105 antibody [MEM-229] (ab69772)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
  • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)

Key features and details

  • Mouse monoclonal [MEM-229] to CD105
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Human, Pig
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

FITC PE PerCP/Cy5.5®

You may also be interested in

Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
Knockout
Product image
Human ENG (CD105) knockout HeLa cell line (ab265178)
Primary
Product image
PE Anti-CD105 antibody [MEM-229] (ab53321)

View more associated products

Overview

  • Product name

    Anti-CD105 antibody [MEM-229]
    See all CD105 primary antibodies
  • Description

    Mouse monoclonal [MEM-229] to CD105
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Pig
  • Immunogen

    Tissue, cells or virus corresponding to Human CD105. Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA

  • Positive control

    • Kg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    MEM-229
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)
    • FITC Anti-CD105 antibody [MEM-229] (ab53318)
    • PE Anti-CD105 antibody [MEM-229] (ab53321)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant human CD105 protein (ab54338)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/200. Fix with Acetone.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-Fr
1/200. Fix with Acetone.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P17813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2022 Human
    • Omim: 131195 Human
    • SwissProt: P17813 Human
    • Unigene: 76753 Human
    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)

      ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5µg/mL. DAPI (blue) was used as nuclear counterstain. 

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab69772? Please let us know so that we can cite the reference in this datasheet.

    ab69772 has been referenced in 10 publications.

    • Cai W  et al. PERK-eIF2a-ERK1/2 axis drives mesenchymal-endothelial transition of cancer-associated fibroblasts in pancreatic cancer. Cancer Lett 515:86-95 (2021). PubMed: 34052329
    • Kaaij MH  et al. Anti-IL-17A treatment reduces serum inflammatory, angiogenic and tissue remodeling biomarkers accompanied by less synovial high endothelial venules in peripheral spondyloarthritis. Sci Rep 10:21094 (2020). PubMed: 33273664
    • Yang Q  et al. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates. Front Bioeng Biotechnol 7:155 (2019). PubMed: 31355191
    • Grau-Vorster M  et al. HLA-DR expression in clinical-grade bone marrow-derived multipotent mesenchymal stromal cells: a two-site study. Stem Cell Res Ther 10:164 (2019). PubMed: 31196185
    • Hong D  et al. Gremlin1 Delivered by Mesenchymal Stromal Cells Promoted Epithelial-Mesenchymal Transition in Human Esophageal Squamous Cell Carcinoma. Cell Physiol Biochem 47:1785-1799 (2018). PubMed: 29953975
    • Calle A  et al. Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation. Stem Cell Res Ther 9:178 (2018). PubMed: 29973295
    • Maruya Y  et al. Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis. Regen Ther 7:24-33 (2017). PubMed: 30271849
    • Kaibuchi N  et al. Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation. Regen Ther 6:83-89 (2017). PubMed: 30271842
    • Liu Y  et al. Characteristics and neural-like differentiation of mesenchymal stem cells derived from foetal porcine bone marrow. Biosci Rep 33:e00032 (2013). PubMed: 23458182
    • Singh S  et al. Mesenchymal stem cells show radioresistance in vivo. J Cell Mol Med 16:877-87 (2012). PubMed: 21762375

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I am using several of your antibodies for immunocytochemistry.  Can send me the catalog numbers of the best isotype controls to use with them and any other technical advice? catalog # ab5694 ab69772 ab19898 Thank you for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 18 2011

    Answer

    Thank you for contacting us. I recommend the following isotype controls for each of these antibodies: ab5694 - Rabbit IgG ab27478 Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html). ab69772 - Mouse IgG2a ab18415 Click here (or use the following: https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18415.html). ab19898 - Rabbit IgG ab27478 Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice.

    Read More

    Abcam Scientific Support

    Answered on Aug 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.