Anti-CD105 antibody [MEM-229] (ab69772)

Submit a review Q&A (1)References (10)
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
  • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)

Key features and details

  • Mouse monoclonal [MEM-229] to CD105
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Human, Pig
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

FITC PE PerCP/Cy5.5®

Overview

  • Product name

    Anti-CD105 antibody [MEM-229]
    See all CD105 primary antibodies

  • Description

    Mouse monoclonal [MEM-229] to CD105

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Human, Pig

  • Immunogen

    Tissue, cells or virus corresponding to Human CD105. Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA

  • Positive control

    • Kg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/200. Fix with Acetone.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-Fr
1/200. Fix with Acetone.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.

  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.

  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)

      ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5µg/mL. DAPI (blue) was used as nuclear counterstain. 

    References (10)

    Publishing research using ab69772? Please let us know so that we can cite the reference in this datasheet.

    ab69772 has been referenced in 10 publications.

    • Cai W  et al. PERK-eIF2a-ERK1/2 axis drives mesenchymal-endothelial transition of cancer-associated fibroblasts in pancreatic cancer. Cancer Lett 515:86-95 (2021). PubMed: 34052329
    • Kaaij MH  et al. Anti-IL-17A treatment reduces serum inflammatory, angiogenic and tissue remodeling biomarkers accompanied by less synovial high endothelial venules in peripheral spondyloarthritis. Sci Rep 10:21094 (2020). PubMed: 33273664
    • Yang Q  et al. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates. Front Bioeng Biotechnol 7:155 (2019). PubMed: 31355191
    • Grau-Vorster M  et al. HLA-DR expression in clinical-grade bone marrow-derived multipotent mesenchymal stromal cells: a two-site study. Stem Cell Res Ther 10:164 (2019). PubMed: 31196185
    • Hong D  et al. Gremlin1 Delivered by Mesenchymal Stromal Cells Promoted Epithelial-Mesenchymal Transition in Human Esophageal Squamous Cell Carcinoma. Cell Physiol Biochem 47:1785-1799 (2018). PubMed: 29953975
    • Calle A  et al. Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation. Stem Cell Res Ther 9:178 (2018). PubMed: 29973295
    • Maruya Y  et al. Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis. Regen Ther 7:24-33 (2017). PubMed: 30271849
    • Kaibuchi N  et al. Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation. Regen Ther 6:83-89 (2017). PubMed: 30271842
    • Liu Y  et al. Characteristics and neural-like differentiation of mesenchymal stem cells derived from foetal porcine bone marrow. Biosci Rep 33:e00032 (2013). PubMed: 23458182
    • Singh S  et al. Mesenchymal stem cells show radioresistance in vivo. J Cell Mol Med 16:877-87 (2012). PubMed: 21762375

    Customer reviews and Q&As

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    Question

    I am using several of your antibodies for immunocytochemistry.  Can send me the catalog numbers of the best isotype controls to use with them and any other technical advice? catalog # ab5694 ab69772 ab19898 Thank you for your help.

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    Answer

    Thank you for contacting us. I recommend the following isotype controls for each of these antibodies: ab5694 - Rabbit IgG ab27478 Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html). ab69772 - Mouse IgG2a ab18415 Click here (or use the following: https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18415.html). ab19898 - Rabbit IgG ab27478 Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice.

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