Anti-CD105 antibody [MEM-229] (ab69772)

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Overview

  • Product name
    Anti-CD105 antibody [MEM-229]
    See all CD105 primary antibodies
  • Description
    Mouse monoclonal [MEM-229] to CD105
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Tissue, cells or virus corresponding to Human CD105.

  • Positive control
    • Kg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab69772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200. Fix with Acetone.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 71 kDa.

Target

  • Function
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)

      ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
      Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5µg/mL. DAPI (blue) was used as nuclear counterstain. 

    References

    ab69772 has not yet been referenced specifically in any publications.

    Publishing research using ab69772? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    I am using several of your antibodies for immunocytochemistry.  Can send me the catalog numbers of the best isotype controls to use with them and any other technical advice? catalog # ab5694 ab69772 ab19898 Thank you for your help.

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    Answer

    Thank you for contacting us. I recommend the following isotype controls for each of these antibodies: ab5694 - Rabbit IgG ab27478 Click here (or use the following: https://www.abcam.com/index.html?datasheet=27478). ab69772 - Mouse IgG2a ab18415 Click here (or use the following: https://www.abcam.com/index.html?datasheet=18415). ab19898 - Rabbit IgG ab27478 Click here (or use the following: https://www.abcam.com/index.html?datasheet=27478). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice.

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