Overview

  • Product name
    Anti-CD105 antibody [MEM-229] (Phycoerythrin)
    See all CD105 primary antibodies
  • Description
    Mouse monoclonal [MEM-229] to CD105 (Phycoerythrin)
  • Host species
    Mouse
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Recombinant vaccinia virus containing human CD105 (L-isoform) cDNA

Properties

Applications

Our Abpromise guarantee covers the use of ab53321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 71 kDa.
IHC-Fr 1/200. Fix with acetone.
ICC Use at an assay dependent concentration.
Flow Cyt Use 20µl for 106 cells.

(or 100µl of whole blood).

 

 

 

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Images

  • ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.

References

This product has been referenced in:
  • Burmeister DM  et al. Delivery of Allogeneic Adipose Stem Cells in Polyethylene Glycol-Fibrin Hydrogels as an Adjunct to Meshed Autografts After Sharp Debridement of Deep Partial Thickness Burns. Stem Cells Transl Med 7:360-372 (2018). ICC/IF ; Pig . Read more (PubMed: 29457376) »
  • Santos AC  et al. Cochlear epithelial of dog fetuses: a new source of multipotent stem cells. Cytotechnology 69:179-189 (2017). Read more (PubMed: 28074389) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your phone call.

Attached please find the list of our antibodies that have been validated for use in Flow Cytometry on sheep cells.

If you are interested in testing one of our other antibodies for use in sheep or Flow Cytometry, I can offer a discount off a future purchase if you buy the antibody now, test it insheep or Flow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me knowwhich antibody you wouldlike to test. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchasethe untested antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it in sheep or Flow Cytometry.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for sheep or Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for contacting us.

The IgG concentration of lot GR37350 is 60ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. I am afraid that according to the supplier of the product, the exact concentration for this product is proprietary and can not be released. The only information they will provide is the number of tests and dilution factor to use. I know that this is not much information for you to get by with and it would be difficult to standardize your experiments. I can only suggest to try callibrating the amount of antibody used with the intensity of the staining, and compare with other antibodies. I am sorry I can't be of more assistance in this occasion, but if there is anything else that I can help you with, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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