Question (21198) | Anti-CD105 antibody [MEM-229] (Phycoerythrin) (ab53321)

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Question

Greetings! I have purchased this antibody and I need to know what the concentration is please. I am conducting multiple-antibody flow cytometry and I need to balance the antibody concentrations, but there is no concentration information on this product, just number of "tests". Thank you!

Answer

Thank you for your enquiry. I am afraid that according to the supplier of the product, the exact concentration for this product is proprietary and can not be released. The only information they will provide is the number of tests and dilution factor to use. I know that this is not much information for you to get by with and it would be difficult to standardize your experiments. I can only suggest to try callibrating the amount of antibody used with the intensity of the staining, and compare with other antibodies. I am sorry I can't be of more assistance in this occasion, but if there is anything else that I can help you with, please do not hesitate to contact me.

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