Anti-CD105 antibody [OTI8A1] (ab156756)
Key features and details
- Mouse monoclonal [OTI8A1] to CD105
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-CD105 antibody [OTI8A1]
See all CD105 primary antibodies -
Description
Mouse monoclonal [OTI8A1] to CD105 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human CD105.
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Positive control
- WB: HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell lysates. IF: HeLa cells. Flow Cyt (Intra): U937 cells.
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General notes
The clone number has been updated from 8A1 to OTI8A1, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab156756 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI8A1 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/4000. Predicted molecular weight: 70 kDa.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/1000.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/200 - 1/4000. Predicted molecular weight: 70 kDa. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/1000. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2022 Human
- Entrez Gene: 13805 Mouse
- Entrez Gene: 497010 Rat
- Omim: 131195 Human
- SwissProt: P17813 Human
- SwissProt: Q63961 Mouse
- Unigene: 76753 Human
- Unigene: 225297 Mouse
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Overlay histogram showing U937 cells stained with ab156756 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab156756, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunofluorescent analysis of HeLa cells labeling CHN1 with ab156756 at 1/100.
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All lanes : Anti-CD105 antibody [OTI8A1] (ab156756) at 1/200 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY CD105
Lysates/proteins at 5 µg per lane.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211854) -
All lanes : Anti-CD105 antibody [OTI8A1] (ab156756) at 1/200 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SVT2 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : COS7 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MDCK cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : MCF7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211696)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab156756 has been referenced in 15 publications.
- Niu W et al. Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix. Biomedicines 9:N/A (2021). PubMed: 33671438
- Guillamat-Prats R et al. Alveolar Type II Cells or Mesenchymal Stem Cells: Comparison of Two Different Cell Therapies for the Treatment of Acute Lung Injury in Rats. Cells 9:N/A (2020). PubMed: 32751857
- Kafarnik C et al. Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro. Stem Cells Dev 29:425-439 (2020). PubMed: 31973649
- Alshihri A et al. The effects of shock wave stimulation of mesenchymal stem cells on proliferation, migration, and differentiation in an injectable gelatin matrix for osteogenic regeneration. J Tissue Eng Regen Med 14:1630-1640 (2020). PubMed: 32885906
- Zhu S et al. Bidirectional ephrinB2-EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells. Int J Mol Med 45:897-909 (2020). PubMed: 31985015