Overview

  • Product name
    Anti-CD105 antibody [SN6]
    See all CD105 primary antibodies
  • Description
    Mouse monoclonal [SN6] to CD105
  • Host species
    Mouse
  • Specificity
    ab11414 recognises the human CD105 cell surface antigen.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WB, ICC/IF, IHC-FoFr, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CD105.

  • Positive control
    • FC: KG1 cells.
  • General notes
    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use 10ul of the neat dilution to label 106 cells in 100ul.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 75 kDa. CD105 is a glycoprotein homodimer of 95kD subunits.
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.

Target

  • Function
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Images

  • ab11414 staining CD105 in human KG1 cells by Flow Cytometry analysis.
  • ab11414 staining CD105 in Human breast tissue by Immunohistochemistry (Frozen sections). The sections were fixed in methanol prior to blocking with 10% serum for 5 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. An HRP-conjugated goat anti-mouse polyclonal was used as the secondary antibody.
    Stains exclusively in the stroma - endothelium of the vessels - in human breast tissue. The image shows epithelial breast gland tissue surrounded by stroma.

    See Abreview

  • IHC - Wholemount of rat brain postnatal sections labelling CD105 with ab11414. Sample was incubated with primary antibody (1/200) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated rabbit anti-mouse IgG monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Yao Y  et al. Synovial fluid-derived synovial fragments represent an improved source of synovial mesenchymal stem cells in the temporomandibular joint. Int J Mol Med 41:173-183 (2018). Read more (PubMed: 29115378) »
  • Wang H  et al. The human umbilical cord stem cells improve the viability of OA degenerated chondrocytes. Mol Med Rep 17:4474-4482 (2018). Read more (PubMed: 29328479) »
See all 26 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Rat Tissue (brain postnatal sections)
Specification
brain postnatal sections

Dr. nic savaskan

Verified customer

Submitted Mar 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH, Neutral, Low pH
Permeabilization
Yes - 0.25% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton
Blocking step
Goat serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 02 2012

Question
Answer

Thank you very much for your calls today and for letting us know about the trouble with ab44967 and these other CD105 antibodies.

As we discussed, I'm sending a free of charge vial of ab11414 on the order ***, which should arrive tomorrow. Please keep me updated about the results with this new antibody, and let me know if there is anything else that we can do for you.

Have a nice day!

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab11414andab225(different lots). The order will ship on Monday for delivery on Tuesday.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

DISCOUNT CODE: XXXXXXXXXXXXXXX
Expiration date: 18-08-2012

DISCOUNT CODE: XXXXXXXXXXXXXXX
Expiration date: 18-08-2012

I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Sheep] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Adipose and bone marrow stem cells)
Specification
Adipose and bone marrow stem cells
Fixative
Paraformaldehyde
Permeabilization
Yes - TRITON x100 0,1%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

Ms. Vega Villar

Verified customer

Submitted Mar 29 2012

Answer

Thank you for your reply.

For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may need to be optimized individually. If there is high background staining, then 2 to 5% blocking agent can also be included.

You may like to consider reviewing our online protocols from our Scientific support protocols and troubleshooting pages. IHC documents are under Immunostaining. These will contain some further information that I hope may be helpful:

https://www.abcam.com/index.html?pageconfig=popular_protocols

Should you have any further questions, please do not hesitate to contact us.

Read More

Question

                    Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti-CD200 antibody      unconjugated    ab17225 Anti-CD146 antibody      PE                           ab78488 Anti-CD34 antibody         unconjugated    ab6330 Anti-CD71 antibody         unconjugated    ab117720 Anti-CD24 antibody         FITC                       ab30350 Anti-CD73 antibody         unconjugated    ab81720                                                 PE                           ab106697 Anti-CD105 antibody      PE                           ab91238                                                 Unconjugated   ab11414 Anti-Cd45 antibody         PE                           ab90960 Anti-STRO1 antibody      unconjugated    ab102969 Anti-CD90 antibody         unconjugated    ab23894                                                 FITC                       ab11155 Anti-CD14 antibody         FITC                       ab86896               Anti-cytokeratin 15         unconjugated    ab1385 IgG2a Isotype control     FITC                       ab81197   Looking forward to your soonest reply. Thank you.   Best regards, Penny Hor BITA LIFESCIENCE SDN. BHD. C-23-05, Dataran 32, No. 2, Jalan 19/1, 46300 Petaling Jaya, Selangor Darul Ehsan, Malaysia t +603 7960 8286    e info@bitalifesc.com.my f +603 7960 8262     

Read More
Answer

Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link: http://www.ncbi.nlm.nih.gov/pubmed/3116408 Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408 Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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