Overview

  • Product name

  • Description

    Rabbit polyclonal to CD109
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD109 aa 920-970 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    GCGEQNMINFA PNIYILDYLT KKKQLTDNLK EKALSFMRQG YQRELLYQRE


    Database link: Q6YHK3

  • Positive control

    • Human lung carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 0.01% BSA

    Aqueous buffered solution
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 162 kDa.
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Target

  • Function

    Modulates negatively TGFB1 signaling in keratinocytes.
  • Tissue specificity

    Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
  • Sequence similarities

    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Post-translational
    modifications

    N-glycosylated.
    2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 150 KDa TGF beta 1 binding protein antibody
    • 150 KDa TGF-beta-1-binding protein antibody
    • 7D1 antibody
    • 8A3 antibody
    • Activated T cell marker CD109 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody
    • CD 109 antibody
    • CD109 antibody
    • CD109 antigen antibody
    • CD109_HUMAN antibody
    • CPAMD7 antibody
    • DKFZp762L1111 antibody
    • E123 antibody
    • FLJ38569 antibody
    • FLJ41966 antibody
    • Gov system alloantigens on platelets antibody
    • p180 antibody
    • Platelet specific Gov antigen antibody
    • Platelet-specific Gov antigen antibody
    • r150 antibody
    • RP11 525G3.1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung carcinoma tissue labeling CD109 with ab203588 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

References

ab203588 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde

Dr. Sébastien Brot

Verified customer

Submitted Jul 09 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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