Key features and details
- Rabbit polyclonal to CD109
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD109 antibody
See all CD109 primary antibodies
DescriptionRabbit polyclonal to CD109
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CD109 aa 967-1122.
Database link: Q6YHK3
- IHC-P: Human placenta tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionModulates negatively TGFB1 signaling in keratinocytes.
Tissue specificityWidely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
Cellular localizationCell membrane.
- Information by UniProt
- 150 KDa TGF beta 1 binding protein antibody
- 150 KDa TGF-beta-1-binding protein antibody
- 7D1 antibody
Paraffin-embedded human placenta tissue stained for CD109 using ab235896 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CD109 (green) using ab235896 at 1/66 dilution in ICC/IF, followed by an Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235896 has not yet been referenced specifically in any publications.