Overview

  • Product name

  • Description

    Rabbit polyclonal to CD109
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD109 aa 967-1122.
    Database link: Q6YHK3

  • Positive control

    • IHC-P: Human placenta tissue. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Function

    Modulates negatively TGFB1 signaling in keratinocytes.
  • Tissue specificity

    Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
  • Sequence similarities

    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Post-translational
    modifications

    N-glycosylated.
    2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 150 KDa TGF beta 1 binding protein antibody
    • 150 KDa TGF-beta-1-binding protein antibody
    • 7D1 antibody
    • 8A3 antibody
    • Activated T cell marker CD109 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody
    • CD 109 antibody
    • CD109 antibody
    • CD109 antigen antibody
    • CD109_HUMAN antibody
    • CPAMD7 antibody
    • DKFZp762L1111 antibody
    • E123 antibody
    • FLJ38569 antibody
    • FLJ41966 antibody
    • Gov system alloantigens on platelets antibody
    • p180 antibody
    • Platelet specific Gov antigen antibody
    • Platelet-specific Gov antigen antibody
    • r150 antibody
    • RP11 525G3.1 antibody
    see all

Images

  • Paraffin-embedded human placenta tissue stained for CD109 using ab235896 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CD109 (green) using ab235896 at 1/66 dilution in ICC/IF, followed by an Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

     

References

ab235896 has not yet been referenced specifically in any publications.

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