Recombinant Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (ab280956)
![Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (ab280956)](https://www.abcam.com/ps/products/280/ab280956/Images/ab280956-2-anti-cd116-antibody-epr2455426-flow-cytometry-mouse-blood-cells.jpg "Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (ab280956)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24554-26] to CD116 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD116 antibody [EPR24554-26] - BSA and Azide free
See all CD116 primary antibodies -
Description
Rabbit monoclonal [EPR24554-26] to CD116 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC,IHC-Fr,IHC-P or WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Mouse blood cells.
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General notes
Please Note: Clone [EPR24554-26] (this product) is different to that of [EPR24554-113] (ab280357) for the same target.
ab280956 is the carrier-free version of ab280951.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24554-26 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. -
Involvement in disease
Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) [MIM:300770]. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12982 Mouse
- SwissProt: Q00941 Mouse
- Unigene: 287228 Mouse
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Alternative names
- CD_antigen=CD116 antibody
- CD116 antibody
- CD116 antigen antibody
see all
Images
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Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (ab280956)
This data was developed using ab280951, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse blood cells cells labelling CD116 with ab280951 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Expression of GMRa is restrcted to cells of the myeloid, but not lymphoid (PMID 17694571).Gated on viable cells.
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![Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (ab280956)](https://www.abcam.com/ps/products/280/ab280956/Images/ab280956-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (ab280956)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab280956 has not yet been referenced specifically in any publications.
