Key features and details
- Rabbit polyclonal to CD11a
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD11a antibody
See all CD11a primary antibodies
DescriptionRabbit polyclonal to CD11a
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human CD11a aa 26-306. The exact sequence is proprietary.
Database link: P20701
- WB: Jurkat and HepG2 cell lysates, Mouse liver tissue lysate; IHC: human appendix, mouse spleen and rat spleen tissues; ICC/ IF: Rat spleen tissue and THP-1 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 129 kDa.|
|IHC-P||1/50 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
Paraffin-embedded human appendix stained for CD11a using ab186873 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of rat spleen stained for CD11a with ab186873 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-CD11a antibody (ab186873) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Mouse liver tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 129 kDa
Exposure time: 60 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Paraffin-embedded mouse spleen tissue stained for CD11a using ab186873 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence staining of THP-1 cells stained for CD11a with ab186873 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
Paraffin-embedded rat spleen tissue stained for CD11a using ab186873 at 1/100 dilution in immunohistochemical analysis.
ab186873 has been referenced in 1 publication.
- Tang TT et al. Employing Macrophage-Derived Microvesicle for Kidney-Targeted Delivery of Dexamethasone: An Efficient Therapeutic Strategy against Renal Inflammation and Fibrosis. Theranostics 9:4740-4755 (2019). PubMed: 31367254