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    cd11a-antibody-ab236245.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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Anti-CD11a antibody (ab236245)

  • Datasheet
  • SDS
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Western blot - Anti-CD11a antibody (ab236245)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab236245)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab236245)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody (ab236245)

Key features and details

  • Rabbit polyclonal to CD11a
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CD11a antibody
    See all CD11a primary antibodies
  • Description

    Rabbit polyclonal to CD11a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD11a aa 201-500.
    Database link: P20701

  • Positive control

    • WB: A549 and HepG2 whole cell lysates. IHC-P: Human tonsil and spleen tissues. ICC/IF: HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Erythrocytic Lineage

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HepG2 whole cell lysate (ab166833)
    • A549 whole cell lysate (ab7910)

Applications

Our Abpromise guarantee covers the use of ab236245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 128 kDa.

Target

  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificity

    Leukocytes.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P20701 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3683 Human
    • Omim: 153370 Human
    • SwissProt: P20701 Human
    • Unigene: 174103 Human
    • Alternative names

      • Lymphocyte function associated antigen, type 1, alpha subunit antibody
      • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
      • Antigen CD11A antibody
      • CD 11a antibody
      • CD11 antigen-like family member A antibody
      • CD11a antibody
      • CD11a antigen antibody
      • Integrin Alpha L antibody
      • Integrin alpha-L antibody
      • Integrin gene promoter antibody
      • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
      • ITAL_HUMAN antibody
      • Itgal antibody
      • ITGAL protein antibody
      • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
      • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
      • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
      • Leukocyte function associated molecule 1 alpha chain antibody
      • Leukocyte function-associated molecule 1 alpha chain antibody
      • LFA 1 alpha (LFA1A) antibody
      • LFA 1 alpha antibody
      • LFA 1 antibody
      • LFA 1A antibody
      • LFA-1A antibody
      • LFA1A antibody
      • Ly15 antibody
      • Ly21 antibody
      • Lymphocyte function associated antigen 1 antibody
      • Lymphocyte Function Associated Antigen Type 1 alpha antibody
      • lymphocyte function-associated antigen 1, alpha polypeptide antibody
      • p180 antibody
      see all

    Images

    • Western blot - Anti-CD11a antibody (ab236245)
      Western blot - Anti-CD11a antibody (ab236245)
      All lanes : Anti-CD11a antibody (ab236245) at 1/500 dilution

      Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size: 128 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab236245)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab236245)

      Paraffin-embedded human tonsil tissue stained for CD11a using ab236245 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab236245)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody (ab236245)

      Paraffin-embedded human spleen tissue stained for CD11a using ab236245 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody (ab236245)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody (ab236245)

      HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CD11a (green) using ab236245 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab236245? Please let us know so that we can cite the reference in this datasheet.

    ab236245 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab236245.
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