Name: Anti-CD11a antibody [EP1285Y]
Brand: RabMAb
SKU: ab52895

Product name

Anti-CD11a antibody [EP1285Y]
See all CD11a primary antibodies
Description

Rabbit monoclonal [EP1285Y] to CD11a
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human, Cynomolgus monkey
Immunogen

Synthetic peptide within Human CD11a aa 1150 to the C-terminus. The exact sequence is proprietary.
Database link: P20701
Positive control
- WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1285Y
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab52895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-Fr		Use at an assay dependent concentration. PubMed: 22319587
WB		1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
IP		1/50.
Flow Cyt		1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100.

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Tissue specificity

Leukocytes.
Sequence similarities

Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Domain

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Cellular localization

Membrane.
Target information above from: UniProt accession P20701 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3683 Human
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
Alternative names
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
- CD 11a antibody
- CD11 antigen-like family member A antibody
- CD11a antibody
- CD11a antigen antibody
- Integrin Alpha L antibody
- Integrin alpha-L antibody
- Integrin gene promoter antibody
- Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
- ITAL_HUMAN antibody
- Itgal antibody
- ITGAL protein antibody
- Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
- Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
- Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
- Leukocyte function associated molecule 1 alpha chain antibody
- Leukocyte function-associated molecule 1 alpha chain antibody
- LFA 1 alpha (LFA1A) antibody
- LFA 1 alpha antibody
- LFA 1 antibody
- LFA 1A antibody
- LFA-1A antibody
- LFA1A antibody
- Ly15 antibody
- Ly21 antibody
- Lymphocyte function associated antigen 1 antibody
- Lymphocyte Function Associated Antigen Type 1 alpha antibody
- lymphocyte function-associated antigen 1, alpha polypeptide antibody
- p180 antibody
see all

Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)

Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).
Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] (ab52895)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)

Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)

Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Immunohistochemistry (Frozen sections) - Anti-CD11a antibody [EP1285Y] (ab52895)Image from Carrillo-de Sauvage MA et al., PLoS One. 2012;7(2):e30762. Epub 2012 Feb 2. Fig S5.; doi:10.1371/journal.pone.0030762; February 2, 2012, PLoS ONE 7(2): e30762.

Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with ab52895 at 1/50 dilution.
Western blot - Anti-CD11a antibody [EP1285Y] (ab52895)

All lanes : Anti-CD11a antibody [EP1285Y] (ab52895) at 1/5000 dilution

Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 2 : THP-1 (Human monocytic leukemia cells) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 129 kDa
Additional bands at: 180 kDa (possible glycosylated form)

Blocking and Dilution buffer: 5% NFDM/TBST

The band in THP-1 cells is higher than that in Jurket cells due to the different glycosylation level in different materials.
Immunoprecipitation - Anti-CD11a antibody [EP1285Y] (ab52895)

CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Datasheet

This product has been referenced in:

Healy LD et al. Activated protein C inhibits neutrophil extracellular trap formation in vitro and activation in vivo. J Biol Chem 292:8616-8629 (2017). Read more (PubMed: 28408624) »
Gao L et al. Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion. Elife 5:N/A (2016). Read more (PubMed: 27763263) »

See all 10 Publications for this product

Publishing research using ab52895? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Product code: 52895
Lot number: GR27346-6
Inquiry: I have noticed that you provide a concentration for some of your antibodies whilst others simply have a volume? I am using the following antibodies.. CD11a - ab52895, Lot GR27346-6 CD54 - ab53013 Lot GR10519-3 Which just have a volume, Do you have access to concentration information for these antibodies? Also is this likely to change if I receive vials from a different 'lot' in the future? Thanks,

Abcam community

Verified customer

Asked on Oct 23 2012

Answer

Thank you for contacting us.

The concentration for lot GR27346-6 is XXXX mg/ml.

The conc. for lot GR10519-3 is XXXX mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

Answered on Oct 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-CD11a antibody [EP1285Y] (ab52895)

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