Recombinant Anti-CD11a antibody [EP1285Y] (ab52895)
![Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-233886-anti-cd11a-antibody-ep1285y-flow-cytometry.jpg "Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1285Y] to CD11a
- Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD11a antibody [EP1285Y]
See all CD11a primary antibodies -
Description
Rabbit monoclonal [EP1285Y] to CD11a -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey
-
Immunogen
Synthetic peptide within Human CD11a aa 1150 to the C-terminus. The exact sequence is proprietary.
Database link: P20701 -
Positive control
- WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1285Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
|
| IP |
1/50.
|
|
| Flow Cyt |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa). |
|
IP
1/50. |
|
Flow Cyt
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
Target
-
Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
Tissue specificity
Leukocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3683 Human
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
-
Alternative names
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
Images
-
Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)
Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-251596-anti-cd11a-antibody-ep1285y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] (ab52895)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-233877-anti-cd11a-antibody-ep1285y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-233879-anti-cd11a-antibody-ep1285y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-233875-anti-cd11a-antibody-ep1285y-western-blot.jpg)
Western blot - Anti-CD11a antibody [EP1285Y] (ab52895)All lanes : Anti-CD11a antibody [EP1285Y] (ab52895) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 2 : THP-1 (Human monocytic leukemia cells) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 129 kDa
Additional bands at: 180 kDa (possible glycosylated form)Blocking and Dilution buffer: 5% NFDM/TBST
The band in THP-1 cells is higher than that in Jurket cells due to the different glycosylation level in different materials.
-
![Immunoprecipitation - Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-233887-anti-cd11a-antibody-ep1285y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD11a antibody [EP1285Y] (ab52895)CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
![Anti-CD11a antibody [EP1285Y] (ab52895)](https://www.abcam.com/ps/products/52/ab52895/Images/ab52895-12-benefits-of-recombinant-antibodies.png)
Anti-CD11a antibody [EP1285Y] (ab52895)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (19)
ab52895 has been referenced in 19 publications.
- Fukuta T et al. Leukocyte-Mimetic Liposomes Penetrate Into Tumor Spheroids and Suppress Spheroid Growth by Encapsulated Doxorubicin. J Pharm Sci 110:1701-1709 (2021). PubMed: 33129835
- Lin ZH et al. The effects of estradiol on inflammatory and endothelial dysfunction in rats with preeclampsia. Int J Mol Med 45:825-835 (2020). PubMed: 31985028
- Fukuta T et al. Leukocyte-mimetic liposomes possessing leukocyte membrane proteins pass through inflamed endothelial cell layer by regulating intercellular junctions. Int J Pharm 563:314-323 (2019). PubMed: 30978483
- Meng ZD et al. Effects of RNA interference-mediated E-selectin gene silencing on cell adhesion molecule expression and cell-cell adhesion in vascular endothelial cells in mice with immunologic contact urticaria. J Cell Biochem 120:115-125 (2019). PubMed: 30206961
- Nice JB et al. Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles. Toxins (Basel) 10:N/A (2018). PubMed: 30322160
