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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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RecombinantRabMAb

Recombinant Anti-CD11a antibody [EP1285Y] (ab52895)

  • Datasheet
Reviews (1)Q&A (1)References (20)

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Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] (ab52895)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)
  • Western blot - Anti-CD11a antibody [EP1285Y] (ab52895)
  • Immunoprecipitation - Anti-CD11a antibody [EP1285Y] (ab52895)
  • Anti-CD11a antibody [EP1285Y] (ab52895)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1285Y] to CD11a
  • Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Biotin Carrier Free

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Overview

  • Product name

    Anti-CD11a antibody [EP1285Y]
    See all CD11a primary antibodies
  • Description

    Rabbit monoclonal [EP1285Y] to CD11a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human CD11a aa 1150 to the C-terminus. The exact sequence is proprietary.
    Database link: P20701

  • Positive control

    • WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1285Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Erythrocytic Lineage

Associated products

  • Alternative Versions

    • Biotin Anti-CD11a antibody [EP1285Y] (ab200022)
    • Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
    • Anti-CD11a antibody [EP1285Y] - Low endotoxin, Azide free (ab246701)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
IP
1/50.
Flow Cyt
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
Notes
WB
1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
IP
1/50.
Flow Cyt
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

Target

  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificity

    Leukocytes.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P20701 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3683 Human
    • Omim: 153370 Human
    • SwissProt: P20701 Human
    • Unigene: 174103 Human
    • Alternative names

      • Lymphocyte function associated antigen, type 1, alpha subunit antibody
      • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
      • Antigen CD11A antibody
      • CD 11a antibody
      • CD11 antigen-like family member A antibody
      • CD11a antibody
      • CD11a antigen antibody
      • Integrin Alpha L antibody
      • Integrin alpha-L antibody
      • Integrin gene promoter antibody
      • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
      • ITAL_HUMAN antibody
      • Itgal antibody
      • ITGAL protein antibody
      • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
      • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
      • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
      • Leukocyte function associated molecule 1 alpha chain antibody
      • Leukocyte function-associated molecule 1 alpha chain antibody
      • LFA 1 alpha (LFA1A) antibody
      • LFA 1 alpha antibody
      • LFA 1 antibody
      • LFA 1A antibody
      • LFA-1A antibody
      • LFA1A antibody
      • Ly15 antibody
      • Ly21 antibody
      • Lymphocyte function associated antigen 1 antibody
      • Lymphocyte Function Associated Antigen Type 1 alpha antibody
      • lymphocyte function-associated antigen 1, alpha polypeptide antibody
      • p180 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)
      Flow Cytometry - Anti-CD11a antibody [EP1285Y] (ab52895)

      Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] (ab52895)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] (ab52895)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)

      Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] (ab52895)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-CD11a antibody [EP1285Y] (ab52895)
      Western blot - Anti-CD11a antibody [EP1285Y] (ab52895)
      All lanes : Anti-CD11a antibody [EP1285Y] (ab52895) at 1/5000 dilution

      Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
      Lane 2 : THP-1 (Human monocytic leukemia cells) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 129 kDa
      Additional bands at: 180 kDa (possible glycosylated form)



      Blocking and Dilution buffer: 5% NFDM/TBST

       

      The band in THP-1 cells is higher than that in Jurket cells due to the different glycosylation level in different materials.

    • Immunoprecipitation - Anti-CD11a antibody [EP1285Y] (ab52895)
      Immunoprecipitation - Anti-CD11a antibody [EP1285Y] (ab52895)

      CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Anti-CD11a antibody [EP1285Y] (ab52895)
      Anti-CD11a antibody [EP1285Y] (ab52895)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (20)

    Publishing research using ab52895? Please let us know so that we can cite the reference in this datasheet.

    ab52895 has been referenced in 20 publications.

    • Fukuta T  et al. Leukocyte-Mimetic Liposomes Penetrate Into Tumor Spheroids and Suppress Spheroid Growth by Encapsulated Doxorubicin. J Pharm Sci 110:1701-1709 (2021). PubMed: 33129835
    • Qiu D  et al. Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1 Expression Levels. Front Genet 11:616988 (2020). PubMed: 33414814
    • Lin ZH  et al. The effects of estradiol on inflammatory and endothelial dysfunction in rats with preeclampsia. Int J Mol Med 45:825-835 (2020). PubMed: 31985028
    • Fukuta T  et al. Leukocyte-mimetic liposomes possessing leukocyte membrane proteins pass through inflamed endothelial cell layer by regulating intercellular junctions. Int J Pharm 563:314-323 (2019). PubMed: 30978483
    • Meng ZD  et al. Effects of RNA interference-mediated E-selectin gene silencing on cell adhesion molecule expression and cell-cell adhesion in vascular endothelial cells in mice with immunologic contact urticaria. J Cell Biochem 120:115-125 (2019). PubMed: 30206961
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-CD11a antibody [EP1285Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Activated NK cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% trisglycine)
    Loading amount
    20 µg
    Specification
    Activated NK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: Roo°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Saravanan Nandagopal

    Verified customer

    Submitted Aug 08 2019

    Question

    Product code: 52895
    Lot number: GR27346-6
    Inquiry: I have noticed that you provide a concentration for some of your antibodies whilst others simply have a volume? I am using the following antibodies.. CD11a - ab52895, Lot GR27346-6 CD54 - ab53013 Lot GR10519-3 Which just have a volume, Do you have access to concentration information for these antibodies? Also is this likely to change if I receive vials from a different 'lot' in the future? Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2012

    Answer

    Thank you for contacting us.

    The concentration for lot GR27346-6 is XXXX mg/ml.

    The conc. for lot GR10519-3 is XXXX mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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