RecombinantRabMAb

Recombinant Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

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Flow Cytometry - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
  • Immunoprecipitation - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
  • Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Biotin Unconjugated

Overview

  • Product name

    Anti-CD11a antibody [EP1285Y] - BSA and Azide free
    See all CD11a primary antibodies

  • Description

    Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract

  • General notes

    ab227119 is the carrier-free version of ab52895.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

  • Tissue specificity

    Leukocytes.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Lymphocyte function associated antigen, type 1, alpha subunit antibody
      • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
      • Antigen CD11A antibody
      • CD 11a antibody
      • CD11 antigen-like family member A antibody
      • CD11a antibody
      • CD11a antigen antibody
      • Integrin Alpha L antibody
      • Integrin alpha-L antibody
      • Integrin gene promoter antibody
      • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
      • ITAL_HUMAN antibody
      • Itgal antibody
      • ITGAL protein antibody
      • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
      • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
      • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
      • Leukocyte function associated molecule 1 alpha chain antibody
      • Leukocyte function-associated molecule 1 alpha chain antibody
      • LFA 1 alpha (LFA1A) antibody
      • LFA 1 alpha antibody
      • LFA 1 antibody
      • LFA 1A antibody
      • LFA-1A antibody
      • LFA1A antibody
      • Ly15 antibody
      • Ly21 antibody
      • Lymphocyte function associated antigen 1 antibody
      • Lymphocyte Function Associated Antigen Type 1 alpha antibody
      • lymphocyte function-associated antigen 1, alpha polypeptide antibody
      • p180 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Flow Cytometry - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    • Immunoprecipitation - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunoprecipitation - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      This IHC data was generated using the same anti-CD11a antibody clone, EP1285Y, in a different buffer formulation (cat# ab52895).

      Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab227119? Please let us know so that we can cite the reference in this datasheet.

    ab227119 has been referenced in 8 publications.

    • Gao L  et al. Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion. Elife 5: (2016). PubMed: 27763263
    • Abramowski P  et al. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS One 9:e104692 (2014). WB ; Human . PubMed: 25181038
    • Takahara M  et al. Soluble ICAM-1 secretion and its functional role as an autocrine growth factor in nasal NK/T cell lymphoma cells. Exp Hematol 41:711-8 (2013). PubMed: 23583640
    • Carrillo-de Sauvage MA  et al. CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo. PLoS One 7:e30762 (2012). IHC-Fr ; Cynomolgus monkey . PubMed: 22319587
    • Jerke U  et al. Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J Biol Chem 286:7070-81 (2011). PubMed: 21193407
    • Rains JL & Jain SK Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells. Am J Physiol Endocrinol Metab 301:E298-306 (2011). WB, Blocking ; Human . PubMed: 21540444
    • Okuzaki D  et al. LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood. BMC Clin Pathol 11:9 (2011). WB ; Human . PubMed: 21849019
    • Tanimoto K  et al. Clinical significance of co-expression of CD21 and LFA-1 in B-cell lymphoma. Int J Hematol 89:497-507 (2009). PubMed: 19360456

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