Overview

  • Product name

    Anti-CD11a antibody [EP1285Y] - BSA and Azide free
    See all CD11a primary antibodies
  • Description

    Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cyt, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human CD11a aa 1150 to the C-terminus. The exact sequence is proprietary.
    Database link: P20701

  • Positive control

    • WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract
  • General notes

    ab227119 is the carrier-free version of ab52895 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab227119 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab227119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 22319587
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificity

    Leukocytes.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lymphocyte function associated antigen, type 1, alpha subunit antibody
    • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
    • Antigen CD11A antibody
    • CD 11a antibody
    • CD11 antigen-like family member A antibody
    • CD11a antibody
    • CD11a antigen antibody
    • Integrin Alpha L antibody
    • Integrin alpha-L antibody
    • Integrin gene promoter antibody
    • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
    • ITAL_HUMAN antibody
    • Itgal antibody
    • ITGAL protein antibody
    • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
    • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
    • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
    • Leukocyte function associated molecule 1 alpha chain antibody
    • Leukocyte function-associated molecule 1 alpha chain antibody
    • LFA 1 alpha (LFA1A) antibody
    • LFA 1 alpha antibody
    • LFA 1 antibody
    • LFA 1A antibody
    • LFA-1A antibody
    • LFA1A antibody
    • Ly15 antibody
    • Ly21 antibody
    • Lymphocyte function associated antigen 1 antibody
    • Lymphocyte Function Associated Antigen Type 1 alpha antibody
    • lymphocyte function-associated antigen 1, alpha polypeptide antibody
    • p180 antibody
    see all

Images

  • Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

  • CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

  • Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with ab52895 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

  • This IHC data was generated using the same anti-CD11a antibody clone, EP1285Y, in a different buffer formulation (cat# ab52895).

    Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Gao L  et al. Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion. Elife 5: (2016). Read more (PubMed: 27763263) »
  • Abramowski P  et al. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS One 9:e104692 (2014). WB ; Human . Read more (PubMed: 25181038) »
See all 8 Publications for this product

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