RecombinantRabMAb

Recombinant Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

Submit a review Submit a question References (8)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD11a antibody [EP1285Y] - BSA and Azide free
    See all CD11a primary antibodies

  • Description

    Rabbit monoclonal [EP1285Y] to CD11a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey

  • Immunogen

    Synthetic peptide within Human CD11a aa 1150 to the C-terminus. The exact sequence is proprietary.
    Database link: P20701

  • Positive control

    • WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract

  • General notes

    ab227119 is the carrier-free version of ab52895 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab227119 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab227119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

  • Tissue specificity

    Leukocytes.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Lymphocyte function associated antigen, type 1, alpha subunit antibody
      • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
      • Antigen CD11A antibody
      • CD 11a antibody
      • CD11 antigen-like family member A antibody
      • CD11a antibody
      • CD11a antigen antibody
      • Integrin Alpha L antibody
      • Integrin alpha-L antibody
      • Integrin gene promoter antibody
      • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
      • ITAL_HUMAN antibody
      • Itgal antibody
      • ITGAL protein antibody
      • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
      • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
      • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
      • Leukocyte function associated molecule 1 alpha chain antibody
      • Leukocyte function-associated molecule 1 alpha chain antibody
      • LFA 1 alpha (LFA1A) antibody
      • LFA 1 alpha antibody
      • LFA 1 antibody
      • LFA 1A antibody
      • LFA-1A antibody
      • LFA1A antibody
      • Ly15 antibody
      • Ly21 antibody
      • Lymphocyte function associated antigen 1 antibody
      • Lymphocyte Function Associated Antigen Type 1 alpha antibody
      • lymphocyte function-associated antigen 1, alpha polypeptide antibody
      • p180 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Flow Cytometry - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image shows membrane and cytoplasmic staining on Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      1. ab52895 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    • Immunoprecipitation - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunoprecipitation - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52895).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

      This IHC data was generated using the same anti-CD11a antibody clone, EP1285Y, in a different buffer formulation (cat# ab52895).

      Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)
      Anti-CD11a antibody [EP1285Y] - BSA and Azide free (ab227119)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab227119? Please let us know so that we can cite the reference in this datasheet.

    ab227119 has been referenced in 8 publications.

    • Gao L  et al. Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion. Elife 5: (2016). PubMed: 27763263
    • Abramowski P  et al. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS One 9:e104692 (2014). WB ; Human . PubMed: 25181038
    • Takahara M  et al. Soluble ICAM-1 secretion and its functional role as an autocrine growth factor in nasal NK/T cell lymphoma cells. Exp Hematol 41:711-8 (2013). PubMed: 23583640
    • Carrillo-de Sauvage MA  et al. CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo. PLoS One 7:e30762 (2012). IHC-Fr ; Cynomolgus monkey . PubMed: 22319587
    • Jerke U  et al. Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J Biol Chem 286:7070-81 (2011). PubMed: 21193407
    • Rains JL & Jain SK Hyperketonemia increases monocyte adhesion to endothelial cells and is mediated by LFA-1 expression in monocytes and ICAM-1 expression in endothelial cells. Am J Physiol Endocrinol Metab 301:E298-306 (2011). WB, Blocking ; Human . PubMed: 21540444
    • Okuzaki D  et al. LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood. BMC Clin Pathol 11:9 (2011). WB ; Human . PubMed: 21849019
    • Tanimoto K  et al. Clinical significance of co-expression of CD21 and LFA-1 in B-cell lymphoma. Int J Hematol 89:497-507 (2009). PubMed: 19360456

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab227119.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com