Recombinant Anti-CD11a antibody [hu1124 (Efalizumab)] - Rabbit IgG (Chimeric) (ab275978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [hu1124 (Efalizumab)] to CD11a - Chimeric
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-CD11a antibody [hu1124 (Efalizumab)] - Rabbit IgG (Chimeric)
See all CD11a primary antibodies -
Description
Rabbit monoclonal [hu1124 (Efalizumab)] to CD11a - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human CD11a.
Database link: P20701 -
Positive control
- Flow cyt: Human lymphocytes. IHC-P: Rat spleen tissue.
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General notes
This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
hu1124 (Efalizumab) -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 3 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 3 µg/ml. |
Target
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Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. -
Tissue specificity
Leukocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3683 Human
- Entrez Gene: 308995 Rat
- Omim: 153370 Human
- SwissProt: P20701 Human
- Unigene: 174103 Human
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Alternative names
- Lymphocyte function associated antigen, type 1, alpha subunit antibody
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- Antigen CD11A antibody
see all
Images
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This data was produced using the human IgG1, kappa version of the antibody.
Flow cytometry of human lymphocytes labeling CD11a with the human IgG1, kappa version of the antibody (panel B) compared with an isotype control (panel A) at 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073) and cells analysed on a FACSCanto flow-cytometer.
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This data was produced using the human IgG1, kappa version of the antibody.
Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of the antibody was used to stain samples at a concentration of 3 µg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275978 has not yet been referenced specifically in any publications.